{"title":"犊牛肠碱性磷酸酶活性丝氨酸周围的氨基酸序列","authors":"Lorents Engström","doi":"10.1016/0926-6569(64)90271-8","DOIUrl":null,"url":null,"abstract":"<div><p>The dipeptides Asp-SER<sup>32</sup>P and Ser<sup>32</sup>P-Ala have been isolated from an acid hydrolysate of calf-intestinal alkaline phosphate (orthophosphoric monoester phosphohydrolase, EC 3.I.3.I), which had been <sup>32</sup>P-labelled on a serine residue at the active site, as described earlier. This shows that the amino acid sequence aroun the active serine is ASP-Ser<sup>32</sup>P-Ala. The peptides were identified by using an acid hydrolysate of crytalline ovalbumin the same unballed peptides as reference substances.</p></div>","PeriodicalId":100170,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","volume":"92 1","pages":"Pages 79-84"},"PeriodicalIF":0.0000,"publicationDate":"1964-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6569(64)90271-8","citationCount":"25","resultStr":"{\"title\":\"The amino acid sequence around the reactive serine in calf-intestinal alkaline phosphatase\",\"authors\":\"Lorents Engström\",\"doi\":\"10.1016/0926-6569(64)90271-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The dipeptides Asp-SER<sup>32</sup>P and Ser<sup>32</sup>P-Ala have been isolated from an acid hydrolysate of calf-intestinal alkaline phosphate (orthophosphoric monoester phosphohydrolase, EC 3.I.3.I), which had been <sup>32</sup>P-labelled on a serine residue at the active site, as described earlier. This shows that the amino acid sequence aroun the active serine is ASP-Ser<sup>32</sup>P-Ala. The peptides were identified by using an acid hydrolysate of crytalline ovalbumin the same unballed peptides as reference substances.</p></div>\",\"PeriodicalId\":100170,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects\",\"volume\":\"92 1\",\"pages\":\"Pages 79-84\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6569(64)90271-8\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926656964902718\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926656964902718","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The amino acid sequence around the reactive serine in calf-intestinal alkaline phosphatase
The dipeptides Asp-SER32P and Ser32P-Ala have been isolated from an acid hydrolysate of calf-intestinal alkaline phosphate (orthophosphoric monoester phosphohydrolase, EC 3.I.3.I), which had been 32P-labelled on a serine residue at the active site, as described earlier. This shows that the amino acid sequence aroun the active serine is ASP-Ser32P-Ala. The peptides were identified by using an acid hydrolysate of crytalline ovalbumin the same unballed peptides as reference substances.
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