板蓝花和石竹细胞悬浮培养中乙酰羟基酸合成酶的研究。

Biochemie und Physiologie der Pflanzen Pub Date : 1992-07-01 DOI:10.1016/S0015-3796(11)80003-X

Walter Maier , Brigitte Schumann , Detlef Gröger

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引用次数: 1

摘要

从板蓝花(十字花科)和芦花(芦花科)的细胞悬浮培养中提取乙酰羟基酸合成酶(EC 4.1.3.18)。采用盐沉淀、凝胶过滤和离子交换色谱相结合的方法进行部分纯化。Isatis和Ruta的AHAS表观分子质量分别为82,000和85,000。FAD是AHAS活性的绝对要求。Isatis-AHAS的视Km值为:FAD 6,3 × 10−6 M;TPP 6,3 × 10−6 m;丙酮酸7 × 10−3和6 × 10−3 M(用于Ruta-AHAS)。支链氨基酸和氯磺隆是isatisa - ahas的反馈抑制剂，但Ruta的乙酰羟酸合成酶对缬氨酸、亮氨酸和异亮氨酸不敏感。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acetohydroxyacid Synthase from Cell Suspension Cultures of Isatis tinctoria l. and Ruta graveolens l.

Acetohydroxyacid synthase (EC 4.1.3.18) has been extracted from cell suspension cultures of Isatis tinctoria (Cruciferae) and Ruta graveolens (Rutaceae). A combination of salt precipitation, gel filtration and ion exchange chromatography was used for partial purification. The apparent molecular masses of AHAS were M_r 82,000 and 85,000 for Isatis and Ruta, respectively. FAD was an absolute requirement for AHAS activity. The apparent K_m values of Isatis-AHAS are the following ones: FAD 6,3 × 10⁻⁶ M; TPP 6,3 × 10⁻⁶ M; pyruvate 7 × 10⁻³, and 6 × 10⁻³ M (for Ruta-AHAS).

Branched-chain amino acids and chlorsulfuron are feedback inhibitors for Isatis-AHAS but acetohydroxyacid synthase from Ruta is not sensitive to valine, leucine and isoleucine.

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