编码c型凝集素的cDNA Na - ctl -2在美洲钩虫成虫中的特异表达

A. Loukas, Alan P Brown, D. Pritchard
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引用次数: 14

摘要

c型凝集素(C-TLs)是碳水化合物结合蛋白,对多种生理过程至关重要,包括免疫、毒液诱导的止血和伤口修复。本文描述了Na - ctl -2的克隆,这是一种编码人类钩虫美洲钩虫分泌的C-TL的cDNA。在成虫的mRNA中检测到转录本,但在感染性幼虫中未检测到。该cDNA编码了一个n端分泌信号肽,后面是一个长形C-TL结构域,序列与秀丽隐杆线虫和哺乳动物抗原提呈细胞受体的C-TL样蛋白相似,这表明钩虫可能利用这类凝集素来中断抗寄生虫免疫反应或干扰宿主凝血机制。这是首次报道的全长cDNA编码钩虫凝集素。该蛋白家族在不同线虫属中的异常倾斜表现及其对线虫寄生进化的后续影响进行了讨论。
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Na - ctl -2, a cDNA Encoding a C-type Lectin Expressed Exclusively in Adult Necator americanus Hookworms
C-type lectins (C-TLs) are carbohydrate-binding proteins central to diverse physiological processes including immunity, venom-induced haemostasis and wound repair. Here we describe the cloning of Na - ctl -2, a cDNA encoding a secreted C-TL from the human hookworm Necator americanus. The transcript was detected in mRNA from adult worms but not infective larvae. The cDNA encoded an N-terminal secretory signal peptide followed by a long-form C-TL domain with sequence similarity to C-TL-like proteins from Caenorhabditis elegans and mammalian antigen presenting cell receptors, suggesting that hookworms might utilise this class of lectin to interrupt anti-parasite immune responses or interfere with host clotting mechanisms. This is the first report of a full-length cDNA encoding a lectin from hookworms. The unusually skewed representation of this protein family within different nematode genera and its subsequent impact on the evolution of nematode parasitism is discussed.
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