C. Boutin, J. Plain, R. Jaffiol, R. Déturche, P. Royer
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Dye-substrate interactions evidenced by fluorescence correlation spectroscopy
In this report, we discuss how the nature of the substrate-water interaction may modify the free diffusion of single molecules, then influencing fluorescence correlation spectroscopy measurements. Our results show a strong influence, up to a few micrometers from the interface, of the surface polarity. This effect is assessed through the relative weight of the two dimension diffusion process observed close to the surface.
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