芍药中重金属及次生代谢物的测定

M. Pavlović, Z. Simić, G. Đelić
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摘要

土壤样品和16种不同提取物中金属(Mg、Ca、Ni、Fe、Mn、Zn、Cu)的总量测定了植物提取物中总酚和总黄酮的含量。用原子吸收光谱仪测定了金属的含量。根据土壤中金属浓度平均值排列顺序为:Fe > Ca > Mg > Mn > Zn > Cu > Ni。所有被测金属的土壤浓度均低于最大允许浓度。结果表明,所分析的植物提取物中含有较高的Ni和Ca含量。虽然所研究的物种积累所分析的金属的量不同,但它们不是这些金属的超积累者。用Folin-Ciocalteu试剂测定总酚含量为1.94 ~ P. oreoselinum、超积累、酚类、黄酮类32.38 mg GA/g。植物提取物中总黄酮含量为0.69 ~ 25.83 mg RU/g。考察了金属与提取物中酚类化合物含量的相关性。根据我们的研究结果,在很大程度上,用奥利索林制茶对人是安全的,因为尽管植物器官对金属的吸收是确定的,但茶中重金属的含量并不高。此外,由于有毒金属(Ni)、酚类和类黄酮含量极低,因此适用于制备茶和草药提取物。
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DETERMINATION OF HEAVY METALS AND SECONDARY METABOLITES OF „PEUCEDANUM OREOSELINUM“ (APIACEAE)
The total quantity of metals (Mg, Ca, Ni, Fe, Mn, Zn, Cu) in soil samples and in sixteen different extracts from plant parts of Peucedanum oreoselinum (L.) Moench as well as the content of total phenols and flavonoids in plant extracts was determined. The contents of metals were determined by the atomic absorption spectrometer. Based on the average values of the metal concentration in the soil, they could be arranged in the following sequence: Fe > Ca > Mg > Mn > Zn > Cu > Ni. Soil concentrations of all tested metals were lower than the maximum allowed concentration. The results demonstrated that the analyzed plant extracts contained higher quantities of Ni and Ca. Although the studied species accumulate analyzed metals in different quantities, they are not hyperaccumulators of these metals. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 1.94 to P. oreoselinum, hyperaccumulation, phenols, flavonoids32.38 mg GA/g. The amounts of flavonoids in plant extracts were in range from 0.69 to 25.83 mg RU/g. We examined the correlation of metals and the phenolic compounds content in the extracts. According to our results the use P. oreoselinum for tea preparation is safe to a great extent for people, because in spite of the determined metal absorption by plant organs, the tea does not contain dangerous quantity of heavy metals. Also, it is suitable for the preparation of teas and herbal extracts due to minimal content of toxic metal (Ni), phenols and flavonoids.
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