几种本地酸樱桃亲缘关系的遗传分析使用RAPD标记

I. Berindean, Alexandra Maria Itu
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引用次数: 0

摘要

樱李是一种产于温带气候带的异源四倍体植物。本研究的目的是利用RAPD标记对罗马尼亚不同果园的酸樱桃进行基因型鉴定。利用14条引物对14个酸樱桃品种进行了RAPD分析。经分析,只有6条引物(OPA 04、OPB10、OPA17、OPA20、OPA03和OPB17)具有较好的多态性,其余引物均为单态。树状图根据其遗传和地理来源将品种分开。品种间的遗传相似性显示出良好的基因型多样性,因此可以将我国本土酸樱桃品种作为不同的基因型,用于未来的选育和新品种鉴定。结果也证实了RAPD引物可用于遗传研究和品种评价。
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Genetic Analysis for Relationships between some Native Sour Cherry (Prunus cerasus l.) Using RAPD Markers
Prunus cerasus L. are an allotetraploid species from temperate climatic zone. The aim of this study was to characterize some native sour cherry genotypes from different orchards from Romania, based on RAPD markers. We used 14 primers RAPD to analyze a total of 14 varieties of sour cherries. After analysis, a good level of polymorphism was generated about only six primers (OPA 04, OPB10, OPA17, OPA20, OPA03 and OPB17) the rest of primers were considered monomorphic. The dendrograme separated the cultivars according to their genetic and geographic origin. The genetic similarity among the cultivars showed a good diversity between the genotypes, so we can suggest that our native cultivars of sour cherry can be considered as distinct genotypes for futures breeding programs and new cultivar identification. Results also confirm that the RAPD primers can be used for genetic studies and to evaluate the varieties for breeding programs.
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