越南黄柏子实体:抗氧化剂和α-淀粉酶抑制剂的潜在来源

Thuy Mai Phuong, Hong Do Thi, Mai Le Thi Tuyet, Hoa Le Thi Phuong
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引用次数: 0

摘要

越南黄连子实体甲醇提取物的正己烷、乙酸乙酯和丁醇组分主要由酚类、黄酮类和萜类化合物组成。乙酸乙酯和丁醇馏分中总酚含量较高。乙酸乙酯组分的酚含量最高(532.96±19.95 mg GAE\g),约占组分干重的53%。各组分对自由基DPPH(1,1-二苯基-2-苦味酰肼)的清除能力与其总酚含量呈正相关。乙酸乙酯部分容量最强,IC50值为0.059 mg\mL,相当于抗坏血酸容量的近50%。在浓度为0.2 mg / mL时,其对铁的还原能力比槲皮素和抗坏血酸的还原能力低一半以上。乙酸乙酯也具有显著的α-淀粉酶抑制活性(IC50= 0.38 mg\mL)。结果表明,鲍氏菌子实体是抗氧化和α-淀粉酶抑制化合物的有效来源。
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VIETNAMESE Phellinus baumii FRUITING BODY, A POTENTIAL SOURCE OF ANTIOXIDANT AND α-AMYLASE INHIBITORY AGENTS
n-Hexane, ethyl acetate, and butanol fractions from methanol extract of Vietnamese Phellinus baumii fruiting body consisted of phenolics, flavonoids, and terpenoids. Ethyl acetate and butanol fractions showed a high level of total phenolics. Ethyl acetate fractions possessed the highest phenolic content (532.96 ± 19.95 mg GAE\g), accounting for approximately 53% fraction dry weight. Free radical DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging capacity of all fractions correlated with their total phenolic content. Ethyl acetate fraction exhibited the strongest capacity with an IC50 value of 0.059 mg\mL, equivalent to nearly 50% of ascorbic acid’s capacity. Its ferric reducing power was off more than half of quercetin and acid ascorbic at the concentration of 0.2 mg\mL. Ethyl acetate also had remarkable α-amylase inhibitory activity (IC50= 0.38 mg\mL). The results suggested P. baumii fruiting body as a potent source for antioxidative and α-amylase inhibitory compounds.
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