洋葱(Allium cepa L.)叶绿体DNA物理图谱,显示了光合作用相关基因的位置

H. Katayama, T. Sasakuma, Y. Ogihara
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引用次数: 14

摘要

为了比较单子叶植物叶绿体基因组的基因顺序,我们构建了洋葱叶绿体DNA (cpDNA)的物理图谱。寿南红,2n=16),有四个限制性内切酶PstI, SalI, XhoI和smi。发现葱属植物的cpDNA是一个圆形分子,总大小约为155 kb,包含两个26 kb的反向重复,将分子的其余部分分成小(约16 kb)和大(约86 kb)的单拷贝区域。用同源探针和异源探针对洋葱总cpDNA进行Southern杂交,确定了物理图谱的内切酶识别位点。在洋葱cpDNA物理图谱上定位了16个基因。洋葱cpDNA的基因组结构与烟草cpDNA在基因组大小和基因顺序上相似,但与禾本科植物cpDNA结构不同。
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Physical map of chloroplast DNA of the onion Allium cepa L., showing the location of photosynthesis-related genes
To compare the gene order of chloroplast genome among monocotyledonous plants, we constructed a physical map of chloroplast DNA (cpDNA) of onion (Allium cepa L. cv. Shounan red, 2n=16) with four restriction endonucleases, PstI, SalI, XhoI, and SmaI. The cpDNA of Allium cepa was found to be a circular molecule with a total size of ca. 155 kb and containing two inverted repeats of 26 kb each that disrupt the rest of the molecule into small (ca. 16 kb) and large (ca. 86 kb) single copy regions. The endonuclease recognition sites of the physical map were confirmed by Southern hybridizations of total onion cpDNA with homologous and heterologous probes. 16 genes were localized on the physical map of the onion cpDNA. Genome structure of onion cpDNA was similar in terms of genome size and gene order of cpDNA to that of tobacco cpDNA, differing from the cpDNA structure of gramineous plants.
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