人嘴唇的脱细胞

H. Khan, M. Szarko, P. Butler
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引用次数: 0

摘要

口腔周围组织丢失通常需要手术重建。自体组织移植导致较差的功能和美观效果,异体组织移植需要终身免疫抑制。临床需要一种细胞支架,它可以用患者自己的细胞播种,以产生免疫原性惰性的口周组织替代物。去细胞化的人类嘴唇可能提供了答案。方法:据作者所知,这是第一次人类嘴唇被脱细胞。现有的四种方法被证明可以成功地从肌肉或真皮中移除细胞,并被用于人类嘴唇的脱细胞,以试图确定最佳的方法。结果:四种方案中的三种被证明是成功地实现了唇部的脱细胞,因为这些样品的组织学调查显示整个结构的细胞结构完全丧失。使用渗透冲击和酶促过程的非洗涤剂为基础的方案最好地保存了细胞外基质。它能够维持胶原蛋白和弹性蛋白的微观结构,并保留重要的信号分子,如糖胺聚糖。结论:这种脱细胞支架可能是一种令人兴奋的治疗口腔周围组织丢失的新方法的第一步。
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Decellularization of Human Lip
Introduction: Perioral tissue loss commonly requires surgical reconstruction. Autologous tissue transfer result in poor functional and aesthetic outcomes and allogenous transplantation of tissue requires lifelong immunosuppression. There is a clinical need for a cell scaffold, which could be seeded with the patients own cells to create an immunogenically inert perioral tissue replacement. Decellularized human lip may provide the answer. Methodology: To the authors knowledge this was the first time human lip has been decellularized. Four existing protocols shown to be successful at removing cells from either muscle or dermis were used to decellularize human lip in an attempt to identify an optimal protocol. Results: Three of the four protocols proved to be successful at achieving decellularization of the lip, as histological investigation of these samples showed complete loss of cellular structures for the entire construct. A non-detergent based protocol using osmotic shock and enzymatic processes best preserved the extracellular matrix. It was able to maintain the micro-architecture of collagen and elastin, and retain important signaling molecules such as glycosaminoglycans. Conclusion: This decellularized scaffold developed here may be the first step towards an exciting new treatment for perioral tissue loss.
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