Débora Ferreira Melo Fragoso, L. M. A. Junior, J. R. Carvalho, D. E. Nava, A. P. Damascena, Dirceu Pratissol
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Trichogramma Westwood, 1833 (Hymenoptera, Trichogrammatidae) collected in orchards in Rio Grande do Sul State, Brazil
The collection and identification of the egg parasitoid Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammatidae) is the first step towards a successful application in the field. This is because, in a biological control program, it is necessary to select the parasitoid species most adapted to the cultivation and climatic conditions of the region where they will be used. Therefore, the objective of this work was to collect Trichogramma in orchards in the State of Rio Grande do Sul, aiming to identify it at the species level by the morphological method and to characterize it by the molecular method of the ITS2 region (internal transcribed space) of rDNA (Ribosomal Deoxyribonucleic Acid). The collection was carried out with traps made with micro-tuller fabric, randomly distributed in orchards. The capture occurred at all collection points. The identification resulted in Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae) for all collections. The DNA fragments present in the agarose gel were around 500 bp.
期刊介绍:
Bothalia: African Biodiversity & Conservation is published by AOSIS for the South African National Biodiversity Institute (SANBI) and aims to disseminate knowledge, information and innovative approaches that promote and enhance the wise use and management of biodiversity in order to sustain the systems and species that support and benefit the people of Africa.
The journal was previously published as Bothalia, and had served the South African botanical community since 1921. However the expanded mandate of SANBI necessitated a broader scope for the journal, and in 2014, the subtitle, African Biodiversity & Conservation was added to reflect this change.