西木赤眼蜂,1833(膜翅目,赤眼蜂科)采集于巴西南里奥格兰德州果园

IF 0.8 4区 生物学 Q4 PLANT SCIENCES Bothalia Pub Date : 2021-01-27 DOI:10.21726/ABC.V7I4.20
Débora Ferreira Melo Fragoso, L. M. A. Junior, J. R. Carvalho, D. E. Nava, A. P. Damascena, Dirceu Pratissol
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引用次数: 1

摘要

采集和鉴定Westwood赤眼蜂1833(膜翅目:赤眼蜂科)卵是成功应用于田间的第一步。这是因为,在生物防治计划中,有必要选择最适合其使用地区的栽培和气候条件的拟寄生物种。因此,本研究的目的是在南巴西大德州的果园采集赤眼蜂,通过形态学方法在种水平上对其进行鉴定,并通过rDNA(核糖体脱氧核糖核酸)ITS2区(内部转录空间)的分子方法对其进行表征。收集是用微薄纱织物制成的陷阱进行的,随机分布在果园里。捕获发生在所有收集点。经鉴定,所有收集物均属赤眼蜂,1879(膜翅目:赤眼蜂科)。琼脂糖凝胶中存在的DNA片段约为500 bp。
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Trichogramma Westwood, 1833 (Hymenoptera, Trichogrammatidae) collected in orchards in Rio Grande do Sul State, Brazil
The collection and identification of the egg parasitoid Trichogramma Westwood, 1833 (Hymenoptera: Trichogrammatidae) is the first step towards a successful application in the field. This is because, in a biological control program, it is necessary to select the parasitoid species most adapted to the cultivation and climatic conditions of the region where they will be used. Therefore, the objective of this work was to collect Trichogramma in orchards in the State of Rio Grande do Sul, aiming to identify it at the species level by the morphological method and to characterize it by the molecular method of the ITS2 region (internal transcribed space) of rDNA (Ribosomal Deoxyribonucleic Acid). The collection was carried out with traps made with micro-tuller fabric, randomly distributed in orchards. The capture occurred at all collection points. The identification resulted in Trichogramma pretiosum Riley, 1879 (Hymenoptera: Trichogrammatidae) for all collections. The DNA fragments present in the agarose gel were around 500 bp.
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来源期刊
Bothalia
Bothalia 生物-植物科学
CiteScore
1.70
自引率
0.00%
发文量
12
期刊介绍: Bothalia: African Biodiversity & Conservation is published by AOSIS for the South African National Biodiversity Institute (SANBI) and aims to disseminate knowledge, information and innovative approaches that promote and enhance the wise use and management of biodiversity in order to sustain the systems and species that support and benefit the people of Africa. The journal was previously published as Bothalia, and had served the South African botanical community since 1921. However the expanded mandate of SANBI necessitated a broader scope for the journal, and in 2014, the subtitle, African Biodiversity & Conservation was added to reflect this change.
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