生物打印石墨烯基微珠近红外控制释放间充质干细胞分泌组用于神经再生

IF 6.8 3区 医学 Q1 ENGINEERING, BIOMEDICAL International Journal of Bioprinting Pub Date : 2023-08-04 DOI:10.36922/ijb.1045
G. Perini, V. Palmieri, M. D’Ascenzo, C. Colussi, C. Grassi, G. Friggeri, A. Augello, Li-ying Cui, M. Papi, M. De Spirito
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引用次数: 0

摘要

神经损伤是一种普遍且使人衰弱的疾病,治疗方法有限。近年来,由于人口老龄化和创伤性脑损伤等因素,神经损伤的发生率有所增加。为了解决有效治疗的迫切需要,本研究探索了间充质干细胞(MSCs)分泌组的控制递送,这是一种复杂的生物活性因子混合物,目前正在研究其在神经再生中的潜力。与干细胞本身相比,分泌组具有显著的优势,因为它可以更容易地表征和控制,从而能够精确地调节治疗干预。然而,挑战在于将分泌组特异性地递送到目标解剖区域。为了克服这一限制,我们提出了一种利用近红外(NIR)辐射响应生物打印海藻酸-氧化石墨烯(AGO)微珠的新方法。氧化石墨烯(GO)是一种高度生物相容性的材料,具有独特的性能,包括近红外响应性,可以在近红外照射下控制治疗剂的释放。我们假设AGO微珠可以包封MSCs分泌组,并通过近红外辐射以可控的方式释放。为了验证我们的假设,我们对海马神经元进行了控制性损伤,并将MSCs分泌组包裹在AGO微珠中。随后,应用近红外辐射触发分泌组的释放。我们比较了MSCs分泌组和星形胶质细胞的功效,星形胶质细胞也具有促进神经生长和增殖的能力。我们的研究结果表明,通过非侵入性近红外辐射,AGO微珠控制MSCs分泌组的释放显著促进了神经损伤后神经元的增殖和再生。AGO微珠与传统的给药方法相比具有多种优势,包括对治疗药物的时间、位置和剂量的精确控制。此外,降低免疫原性和致瘤性的潜力增强了该疗法的安全性。因此,本研究为基于msc的神经再生疗法的发展提供了一条有希望的途径,对各种神经病变和损伤的治疗具有重要意义。
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Near-infrared controlled release of mesenchymal stem cells secretome from bioprinted graphene-based microbeads for nerve regeneration
 Nerve damage is a prevalent and debilitating condition with limited treatment options. Recent years have seen an increased incidence of neural damage due to factors such as aging populations and traumatic brain injuries. Addressing the urgent need for effective therapies, this study explores the controlled delivery of mesenchymal stem cells (MSCs) secretome, a complex mixture of bioactive factors, which is currently being investigated for its potential in nerve regeneration. The secretome offers significant advantages over stem cells themselves, as it can be more easily characterized and controlled, enabling precise regulation of therapeutic interventions. However, the challenge lies in delivering the secretome specifically to the target anatomical region. To overcome this limitation, we propose a novel approach utilizing near-infrared (NIR) radiation-responsive bioprinted alginate-graphene oxide (AGO) microbeads. Graphene oxide (GO) is a highly biocompatible material with unique properties, including NIR responsiveness, enabling controlled release of therapeutic agents upon NIR exposure. We hypothesized that AGO microbeads could encapsulate MSCs secretome and release it in a controlled manner using NIR radiation. To investigate our hypothesis, controlled damage was induced to hippocampal neurons, and MSCs secretome was encapsulated within AGO microbeads. Subsequently, NIR radiation was applied to trigger the release of the secretome. We compared the efficacy of MSCs secretome with that of astrocytes, which also possess nerve growth and proliferation-promoting capabilities. Our findings demonstrated that the controlled release of MSCs secretome from AGO microbeads through non-invasive NIR radiation significantly promoted the proliferation and regeneration of neurons following nerve injury. AGO microbeads offer multiple advantages over conventional delivery methods, including precise control over the timing, location, and dosage of therapeutic agents. Furthermore, the potential for reduced immunogenicity and tumorigenicity enhances the safety profile of the therapy. Consequently, this study presents a promising avenue for the development of MSC-based therapies for nerve regeneration, with implications for the treatment of various neuropathies and injuries.  
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来源期刊
CiteScore
6.90
自引率
4.80%
发文量
81
期刊介绍: The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.
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