去卵巢大鼠骨盆骨的红外和拉曼光谱分析

IF 0.8 Q3 MULTIDISCIPLINARY SCIENCES Makara Journal of Science Pub Date : 2022-03-30 DOI:10.7454/mss.v26i1.1284
Dyah Fitriyawardhani, A. Juwono, N. N. Mulyaningsih
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引用次数: 0

摘要

骨质疏松症是一种骨质疾病,通常发生在绝经后妇女。骨质疏松症可以用动物模型来研究,比如大鼠,因为大鼠的骨骼结构与人类的骨骼结构相似。本研究旨在分析首次出现骨质疏松症状的去卵巢大鼠盆腔骨。本研究选取20只褐家鼠在12周龄时进行卵巢切除术,作为绝经期妇女的动物模型。13 ~ 21周龄每2周对大鼠实施安乐死。此外,将使用FTIR和拉曼光谱定期分析去卵巢大鼠的骨盆骨,以显示早期骨质疏松症的迹象。FTIR和拉曼光谱分析结果显示,去卵巢大鼠骨盆骨在17周龄时首次出现骨质疏松症状,磷酸盐和碳酸盐含量改变,羟基官能团增加,矿物成熟度降低约7.47%,结晶度降低约29.26%。SEM-EDS和XRD观察到,在2θ = 25°角处,纤维从细纤维到粗纤维的形态变化,晶粒尺寸和结晶度指数分别降低了9.29%和25.18%。
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Analysis of Pelvic Bone of Ovariectomized Rats using Infrared and Raman Spectroscopies
Osteoporosis is a bone disease that generally occurs in postmenopausal women. Osteoporosis can be studied using an animal model such as rats because rat bone structure is similar to human bone structure. This research aimed to analyze pelvic bones of ovariectomized rats that showed osteoporosis signs for the first time. In this study, 20 Rattus norvegicus were given ovariectomy at 12 weeks of age and were used as an animal model for menopausal women. The rats were euthanized every 2 weeks from 13 to 21 weeks of age. In addition, the pelvic bones of ovariectomized rats will be analyzed using FTIR and Raman spectroscopy to show the first osteoporosis signs periodically. Results of FTIR and Raman spectroscopy indicated that the pelvic bones of the ovariectomized rats showed the first osteoporosis signs at 17 weeks of age with changes in phosphate and carbonate contents, increased hydroxyl functional groups, reduction of mineral maturity about 7.47%, and reduction of crystallinity about 29.26%. In addition, morphological changes from fine fibers to coarser fibers and reduction of the crystal size and crystallinity index at the angle of 2θ ≅ 25° about 9.29% and 25.18%, respectively, were observed using SEM-EDS and XRD.
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来源期刊
Makara Journal of Science
Makara Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.30
自引率
20.00%
发文量
24
审稿时长
24 weeks
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