阿尔巴尼亚小麦(Triticum aestivum L.)的遗传多样性和粗蛋白质含量变异

A. Dervishi, Merita Rumano, Panajota Ruzi, A. Çakalli
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引用次数: 1

摘要

研究了11个小麦基因型的遗传多样性和粗蛋白质含量的变异,其中包括3个优质本地基因型和8个外源小麦基因型。双缩脲试验测定的籽粒蛋白质含量存在差异,其变化范围为9.5 ~ 13.9%,平均值为11.58%。通过与引进小麦基因型的比较分析,发现本地小麦基因型的蛋白质含量低于外源小麦基因型。利用14个多态性RAPD标记对小麦品种的遗传多样性进行了评价。小麦基因型间的平均相似性为67%。本地小麦品种间的遗传相似性(83%)高于外源小麦品种间(66%)。在基于Dice相似系数构建的UPGMA树状图上,将小麦基因型划分为两个主要聚类。根据来源,观察到明显的基因型聚类。主坐标分析(PCoA)结果也支持这种聚类。没有观察到基于蛋白质含量的聚类。结果表明,本地小麦遗传多样性较窄,而外源小麦基因型在提高小麦蛋白质含量和遗传多样性方面具有广阔的应用前景。
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The genetic diversity and variation in crude protein content of wheat (Triticum aestivum L.) promising cultivars for breeding in Albania
The genetic diversity and variation in crude protein content among eleven wheat genotypes, comprising three elite local genotypes and eight wheat genotypes of foreign origin were investigated in the present study. Variability was evidenced in grain protein content estimated by biuret test, it ranged from 9.5 to 13.9% with mean of 11.58%. Comparative analysis between local and introduced wheat genotypes revealed that the local genotypes had lower protein content than those of foreign origin. Fourteen polymorphic RAPD markers were used to assess genetic diversity among selected wheat varieties. The mean similarity among wheat genotypes was 67%. Genetic similarity among local wheat varieties was higher (83%) than among those of foreign origin (66%). The wheat genotypes were grouped into two main clusters on UPGMA dendrogram constructed based on Dice similarity coefficients. A clear clustering of genotypes according to the origin wasobserved. This clustering was also supported by principal coordinate analysis (PCoA) results. There was no observed clustering based on the protein content. The data revealed that local wheat genetic had narrow genetic diversity, however the wheat genotypes of foreign origin constitute a promising material to be employed in breeding programs aiming the increase of wheat protein content and genetic diversity.
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