甘氨酸中异黄酮的蛋白质转化因子和抗氧化能力

IF 0.7 4区 医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2023-09-30 DOI:10.37360/blacpma.23.22.5.47
Edmundo Venegas-Casanova, Jose G Gavidia-Valencia, Yuri F Curo-Vallejos, Miguel Rios, Luis A Chavez-Abanto, Ruben J Aro-Diaz, Jorge Escobar, Felipe Ruben Rubio-Lopez, Jose Uribe-Villarreal, Jose L. Martinez, R. Rengifo-Penadillos
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引用次数: 0

摘要

测定了甘氨酸中氮转化为蛋白质和异黄酮的因子。转换因子的测定,先将蛋白在碱性条件下溶解,再用酸性条件提取,用凯氏定氮法鉴定。先用索氏法提取异黄酮及其苷,再用Brand Williams法(DPPH)测定其抗氧化能力。结果表明:蛋白质转化因子为5.85,总异黄酮最大浓度为33.33%,总异黄酮抗自由基效率为0.004 mL/ugmin,没食子酸抗自由基效率为0.005 mL/ugmin。单宁酸的抗自由基效率为0.0004 mL/ugmin。这些结果证明食用甘氨酸(大豆)是一种具有高营养质量的食物,是抗氧化剂的极好来源,可以预防激素和致癌疾病。
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Protein conversion factor and antioxidant capacityof isoflavones in Glycine max
The conversion factor of nitrogen to proteins and isoflavones present in Glycine maxwas determined. For the determination of the conversion factor, we worked with solubilizing the proteins at alkaline pH and then extracting them with acidic pH. The proteins were identified by the Kjeldahl method. The antioxidant capacity was determined after extracting the isoflavones and their glycosides through the Soxhlet method, and then using the Brand Williams method (DPPH). The results indicate that the protein conversion factor was 5.85, the maximum concentration of total isoflavones was 33.33%, the antiradical efficiency of total isoflavones was 0.004 mL/ugmin, the antiradical efficiency of gallic acid was 0.005 mL/ugmin. and the antiradical efficiency of tannic acid was 0.0004 mL/ugmin. These results justify the consumption of Glycine max(Soya)as a food that has a high nutritional quality and provides an excellent source of antioxidants, which will prevent hormonal and carcinogenic diseases.
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来源期刊
CiteScore
1.30
自引率
14.30%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.
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