纳米工程表面对成骨细胞粘附、生长、分化和凋亡的影响

R. Miralami, J. Sharp, F. Namavar, Curtis W. Hartman, K. Garvin, G. Thiele
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引用次数: 0

摘要

通过增强成骨细胞的激活、生长、分化和诱导更大的骨形成和更强的附着体来改善种植体表面以提高其生物相容性,可以改善全关节置换术的结果。本研究验证了一种假设,即通过离子束辅助沉积方法产生的纳米结构表面可以增强成骨细胞的粘附、生长、分化、骨形成和成熟。采用离子束辅助沉积技术在玻璃衬底上沉积氧化锆薄膜。通过比较离子束辅助沉积技术对人骨肉瘤细胞系SAOS-2在涂膜和未涂膜表面的粘附、增殖、分化和凋亡的影响,研究了离子束辅助沉积技术对细胞功能的影响。与玻璃表面相比,离子束辅助沉积纳米涂层增强了氧化锆纳米涂层表面上4′,6-二氨基-2-苯基吲哚染色细胞核的数量。这种纳米修饰也增加了线粒体脱氢酶活性的细胞增殖。此外,离子束辅助沉积技术通过矿化骨结节的形成和钙沉积的速率来改善细胞分化,这两者都是成功骨形成的体外指标。然而,通过Annexin V染色和流式细胞术评估的程序性细胞死亡在纳米表面和玻璃表面之间没有统计学上的显著差异。总之,研究结果表明,离子束辅助沉积技术制备的纳米氧化锆表面在支持骨细胞粘附、增殖和分化方面优于非涂层表面。因此,离子束辅助沉积技术改变的表面特性增强了骨的形成,并可能增加骨细胞相关表面的生物相容性。
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Effects of nano-engineered surfaces on osteoblast adhesion, growth, differentiation, and apoptosis
Modifying implant surfaces to improve their biocompatibility by enhancing osteoblast activation, growth, differentiation, and induction of greater bone formation with stronger attachments should result in improved outcomes for total joint replacement surgeries. This study tested the hypothesis that nano-structured surfaces, produced by the ion beam-assisted deposition method, enhance osteoblast adhesion, growth, differentiation, bone formation, and maturation. The ion beam-assisted deposition technique was employed to deposit zirconium oxide films on glass substrates. The effects of the ion beam-assisted deposition technique on cellular functions were investigated by comparing adhesion, proliferation, differentiation, and apoptosis of the human osteosarcoma cell line SAOS-2 on coated versus uncoated surfaces. Ion beam-assisted deposition nano-coatings enhanced initial cell adhesion assessed by the number of 4′,6-diamidino-2-phenylindole–stained nuclei on zirconium oxide nano-coated surfaces compared to glass surfaces. This nano-modification also increased cell proliferation as measured by mitochondrial dehydrogenase activity. Moreover, the ion beam-assisted deposition technique improved cell differentiation as determined by the formation of mineralized bone nodules and by the rate of calcium deposition, both of which are in vitro indicators of the successful bone formation. However, programmed cell death assessed by Annexin V staining and flow cytometry was not statistically significantly different between nano-surfaces and glass surfaces. Overall, the results indicate that nano-crystalline zirconium oxide surfaces produced by the ion beam-assisted deposition technique are superior to uncoated surfaces in supporting bone cell adhesion, proliferation, and differentiation. Thus, surface properties altered by the ion beam-assisted deposition technique enhanced bone formation and may increase the biocompatibility of bone cell–associated surfaces.
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来源期刊
CiteScore
6.00
自引率
1.70%
发文量
24
期刊介绍: Proceedings of the Institution of Mechanical Engineers Part N-Journal of Nanomaterials Nanoengineering and Nanosystems is a peer-reviewed scientific journal published since 2004 by SAGE Publications on behalf of the Institution of Mechanical Engineers. The journal focuses on research in the field of nanoengineering, nanoscience and nanotechnology and aims to publish high quality academic papers in this field. In addition, the journal is indexed in several reputable academic databases and abstracting services, including Scopus, Compendex, and CSA's Advanced Polymers Abstracts, Composites Industry Abstracts, and Earthquake Engineering Abstracts.
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