Improving Detection of Somatic Mutations

J Cancer Res Therap Oncol 2019 | Vol 7: 101 Interest in this topic originated from studies on the detection of Braf p. V600E/K mutations using Allele Specific Multiplex Sequencing (ASMS) technology, which was compared to detection of Braf p. V600E by either SNapShot or Ion Torrent. Although there was no discordance among the positives, there was significant discordance among the negatives, indicative of potential false negatives by the latter two methods [1]. A similar pattern was observed when ASMS Braf p. V600E/K mutations was compared to an FDA approved Braf Test (ThxID) (unpublished data). Is there any clinical significance to such discordant results? If so, can testing protocols of somatic mutations be improved to accommodate for potential discrepancies among the test methods so that the results generated could be graded for meaningful clinical interpretation? There are at least four areas that could be affected by such discordant results;