{"title":"迷迭香体外增殖的研究","authors":"H.C. Chaturvedi, Pratibha Misra, Meena Sharma","doi":"10.1016/S0044-328X(84)80035-5","DOIUrl":null,"url":null,"abstract":"<div><p>A method for rapid multiplication of <em>Rosmarinus officinalis</em> L. var. <em>genuina</em> forma <em>erectus</em> was developed affording production of about 5,000 plants from one nodal segment in one year. Plants could be established in culture only with nodal stem segments taken from fieldgrown plants. Ca. 14 shoot buds differentiated per shoot apex, excised from aseptically established plants, in medium with 0.2 mg·l<sup>−1</sup> BAP within 30 days. Isolated shoots rooted 80% in the presence of 0.25 mg·l<sup>−1</sup> IPA in 7 days. The <em>in vitro</em>-raised plants grew normally in soil under glasshouse conditions.</p></div>","PeriodicalId":23797,"journal":{"name":"Zeitschrift für Pflanzenphysiologie","volume":"113 4","pages":"Pages 301-304"},"PeriodicalIF":0.0000,"publicationDate":"1984-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80035-5","citationCount":"7","resultStr":"{\"title\":\"In vitro Multiplication of Rosmarinus officinalis L.)\",\"authors\":\"H.C. Chaturvedi, Pratibha Misra, Meena Sharma\",\"doi\":\"10.1016/S0044-328X(84)80035-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A method for rapid multiplication of <em>Rosmarinus officinalis</em> L. var. <em>genuina</em> forma <em>erectus</em> was developed affording production of about 5,000 plants from one nodal segment in one year. Plants could be established in culture only with nodal stem segments taken from fieldgrown plants. Ca. 14 shoot buds differentiated per shoot apex, excised from aseptically established plants, in medium with 0.2 mg·l<sup>−1</sup> BAP within 30 days. Isolated shoots rooted 80% in the presence of 0.25 mg·l<sup>−1</sup> IPA in 7 days. The <em>in vitro</em>-raised plants grew normally in soil under glasshouse conditions.</p></div>\",\"PeriodicalId\":23797,\"journal\":{\"name\":\"Zeitschrift für Pflanzenphysiologie\",\"volume\":\"113 4\",\"pages\":\"Pages 301-304\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0044-328X(84)80035-5\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift für Pflanzenphysiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0044328X84800355\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift für Pflanzenphysiologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0044328X84800355","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vitro Multiplication of Rosmarinus officinalis L.)
A method for rapid multiplication of Rosmarinus officinalis L. var. genuina forma erectus was developed affording production of about 5,000 plants from one nodal segment in one year. Plants could be established in culture only with nodal stem segments taken from fieldgrown plants. Ca. 14 shoot buds differentiated per shoot apex, excised from aseptically established plants, in medium with 0.2 mg·l−1 BAP within 30 days. Isolated shoots rooted 80% in the presence of 0.25 mg·l−1 IPA in 7 days. The in vitro-raised plants grew normally in soil under glasshouse conditions.
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