V. Maioli, Antoine Boniface, P. Mahou, Júlia Ferrer Ortas, L. Abdeladim, E. Beaurepaire, Willy Supatto
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Multiphoton Light-sheet Microscopy at Optimal Pulse Frequency for Fast In Vivo Imaging
Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase in live two-photon light-sheet imaging.
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