DNA条形码技术在阿联酋沙迦市主要鱼种鉴定中的应用

Alaa Abdelbaset-Donya, W. Hamza, K. Mohammed-Geba, A. Galal-Khallaf
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引用次数: 2

摘要

阿拉伯联合酋长国(UAE)已被确定为一个活跃的国际海鲜市场。为了确定阿联酋市场上的一些关键鱼类,在阿联酋最大的鱼市之一沙迦市的朱拜勒市场随机采集了9种鲜鱼。利用细胞色素氧化酶亚基I (coi)基因的高变5´区域的部分测序对样品进行DNA条形码。此外,对结果进行了系统发育分析,以进一步提供准确的物种鉴定。遗传和系统发育分析解决了在30%的鱼类中发现的命名差异。各物种内部遗传多样性呈现高单倍型多样性的总体趋势。大多数单倍型与阿曼海和阿拉伯湾共享,与红海共享的很少。研究结果强烈建议在阿联酋海鲜市场持续应用基于分子的物种检查,以确保本地物种的真实性、可追溯性和适当保护。
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APPLICATION OF DNA BARCODING FOR IDENTIFICATION OF SOME KEY FISH-MARKET SPECIES IN SHARJAH CITY, UAE
The United Arab Emirates (UAE) has been identified as an active international seafood market. To identify some of the key fish species in the UAE markets, 9 species of fresh fishes were randomly collected from Souq Al Jubail in Sharjah City, one of the biggest fish markets in UAE. The samples were subjected to DNA barcoding using partial sequencing of the hypervariable 5´ region of the cytochrome oxidase subunit I (coi) gene. Furthermore, the results were phylogenetically analyzed to provide further accurate species identification. Genetic and phylogenetic analyses resolved the naming discrepancies found in 30% of the fishes. Genetic diversity within each species exhibited a general trend for high haplotypes´ diversities. Most haplotypes were shared with the Sea of Oman and the Arabian Gulf, and few with the Red Sea. The results strongly recommend continuous application of molecular-based species inspection in the UAE seafood markets for assuring authenticity, traceability, and proper conservation of the native species.
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