{"title":"三次谐波代显微镜下小鼠脑皮层髓鞘无标记活体成像","authors":"Michael J. Redlich, Hyungsik Lim","doi":"10.1364/boda.2019.jt4a.9","DOIUrl":null,"url":null,"abstract":"We demonstrate label-free intravital imaging of the myelinated fibers in the cerebral cortex of mouse by third-harmonic generation microscopy. Using an optical parametric oscillator as the excitation source, the depth of 250 pm is achieved.","PeriodicalId":8973,"journal":{"name":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","volume":"346 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Label-Free Intravital Imaging of Cortical Myelin in Mouse Brain by Third-Harmonic Generation Microscopy\",\"authors\":\"Michael J. Redlich, Hyungsik Lim\",\"doi\":\"10.1364/boda.2019.jt4a.9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We demonstrate label-free intravital imaging of the myelinated fibers in the cerebral cortex of mouse by third-harmonic generation microscopy. Using an optical parametric oscillator as the excitation source, the depth of 250 pm is achieved.\",\"PeriodicalId\":8973,\"journal\":{\"name\":\"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)\",\"volume\":\"346 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-04-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1364/boda.2019.jt4a.9\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/boda.2019.jt4a.9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Label-Free Intravital Imaging of Cortical Myelin in Mouse Brain by Third-Harmonic Generation Microscopy
We demonstrate label-free intravital imaging of the myelinated fibers in the cerebral cortex of mouse by third-harmonic generation microscopy. Using an optical parametric oscillator as the excitation source, the depth of 250 pm is achieved.
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