水玻璃介导番茄抗氧化防御系统的响应。在水分胁迫下

C. Malhotra, R. Kapoor, D. Ganjewala, Nb Singh
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引用次数: 9

摘要

本研究旨在研究水分胁迫对番茄幼苗生长的影响。以及水玻璃在番茄亏水条件下的保护作用。采用标准方法测定了40和60 DAS条件下番茄叶片光合色素、蛋白质、糖、丙二醛含量、脯氨酸、硝酸还原酶活性和抗氧化酶(SOD、CAT、APX和POX)活性等生化指标。测定了60、65和70 DAS时番茄果实中番茄红素和β-胡萝卜素的含量。水分胁迫显著降低了60 DAS下番茄叶片的相对含水量、色素含量、糖和蛋白质含量,但促进了脯氨酸的积累。水分胁迫60 DAS后(3d和6d),抗氧化酶SOD、CAT、APX和POX活性显著升高。本研究为番茄水分胁迫诱导氧化应激及抗旱抗氧化防御系统的建立提供了第一手资料。结果表明,水玻璃对番茄水分亏缺条件下植株的保护具有积极作用。
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Sodium silicate mediated response of antioxidative defense system in Lycopersicon esculentum mill. under water stress
The present study was designed to study the effect of water stress on Lycopersicon esculentum Mill. and role of sodium silicate in the protection of tomato plants under water deficit condition. Different biochemical parameters such as photosynthetic pigments, protein, sugar, MDA content, proline, nitrate reductase activity and activities of antioxidant enzymes (SOD, CAT, APX and POX) were examined in tomato leaves at 40 and 60 DAS by the standard methods. The lycopene and β-carotene contents in tomato fruits were also analyzed at 60, 65 and 70 DAS. Water stress significantly decreased relative water content (RWC), pigment content, sugar and protein contents in tomato leaves at 60 DAS but the accumulation of proline was stimulated in tomato leaves under water deficit condition. The activities of antioxidant enzymes such as SOD, CAT, APX and POX were significantly increased under (3d and 6d) water stress condition at 60 DAS. This study offers first hand information on the water stress-induced oxidative stress in Lycopersicon esculentum and development of antioxidative defense system against drought. The results obtained clearly indicated the positive impact of sodium silicate in protection of tomato plants under water deficit condition.
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