实验性前列腺癌变的区域淋巴结形态

V. Astashov, A. Lomshakov, T. Tsekhmistrenko, O. Vykhristyuk, M. Uloga, Inna Borodina, A. Tolkachev
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引用次数: 0

摘要

背景:肿瘤细胞向血液和淋巴管的迁移是恶性肿瘤转移过程的关键阶段。此外,淋巴结内肿瘤细胞的存在是肿瘤侵袭性的一个指标,淋巴结的组织病理学特征是治疗预后和患者生存的重要参数之一。目的:评价实验性前列腺癌变过程中小鼠淋巴结的变化。材料与方法:选用CBA近交系3月龄雄性小鼠30只。为了建立前列腺恶性肿瘤生长模型,将可移植的埃利希腹水癌稀释细胞株接种到前列腺实质中。将埃利希腹水癌移植至小鼠前列腺组织,于18 d建立前列腺癌模型(n=15;主要组)。其余15只小鼠作为对照组。所有对动物的操作都遵循基本的伦理原则。将动物从实验中取出后,对前列腺和区域(盆腔)淋巴结进行形态学分析。对两组动物盆腔淋巴结主要结构区和功能区的面积和细胞组成进行了评估。结果:本研究表明盆腔淋巴结前列腺癌发生后细胞组成发生了变化。主组动物继发性淋巴结节生发中心的免疫母细胞数量显著高于对照组(p< 0.05)。小鼠(实验组)免疫母细胞增加近2倍,表明前列腺癌发生过程中体液免疫受到抑制。与对照组相比,主组皮质物质面积减少14.5%,副皮质面积增加28.4% (p< 0.05)。结论:前列腺癌的第一次转移发生在区域淋巴结。在前列腺癌变第18天,区域淋巴结结构发生改变:皮质旁增生,髓索及髓质面积减少,脑窦减少。关键词:前列腺癌,癌变,实验,埃利希腹水癌,淋巴管生成,淋巴结,区域转移。引文:Astashov v.v., Lomshakov a.a., Tsekhmistrenko T.A.等。实验性前列腺癌变的区域淋巴结形态。俄罗斯医学调查。2022;6(4):171-176(俄文)。DOI: 10.32364 / 2587-6821-2022-6-4-171-176。
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Morphology of regional lymph nodes in experimental prostate carcinogenesis
Background: the migration of tumor cells into blood and lymphatic vessels is a key stage of the metastatic process in malignant neoplasms. Moreover, the presence of tumor cells in the lymph nodes is an indicator of cancer aggressiveness, and the histopathological characteristics of the lymph nodes are one of the important parameters for the treatment prognosis and patient survival Aim: to evaluate changes in the lymph nodes of mice during experimental carcinogenesis in the prostate gland. Material and Methods: the study was conducted on CBA inbred 3-month-old male mice (n=30). To create a model of malignant tumor growth in the prostate gland, a diluted cell strain of transplantable Ehrlich ascites carcinoma was inoculated into the prostate parenchyma. A model of prostate carcinogenesis was created in 18 days by transplanting Ehrlich ascites carcinoma into prostate tissue in mice (n=15; main group). The remaining 15 mice were in the control group. All manipulations with animals were carried out in compliance with the basic ethical principles. After the animals were removed from the experiment, a morphological analysis of the prostate and regional (pelvic) lymph nodes was performed. The area and cellular composition concerning the main structural and functional zones of the pelvic lymph nodes in both groups of animals were evaluated Results: this study showed that the cellular composition changed in the setting of prostate carcinogenesis in the pelvic lymph nodes. In the main group of animals, the number of immunoblasts significantly prevailed in the germinal center of secondary lymphoid nodules versus the control group (p<0,05). An increase in immunoblasts by almost 2 times in mice (experimental group) indicated the inhibition of humoral immunity in prostate carcinogenesis. Also, in the main group, the area of the cortical substance decreased by 14.5% and the area of the paracortex increased by 28.4% versus the control group (p<0,05). Conclusion: the first metastases of prostate cancer appear in regional lymph nodes. On the 18th day of prostate carcinogenesis, the structure of regional lymph nodes undergoes changes: paracortical hyperplasia, a decrease in the area of medullary cords and medullary substance, a decrease in cerebral sinuses KEYWORDS: prostate cancer, carcinogenesis, experiment, Ehrlich ascites carcinoma, lymphangiogenesis, lymph node, regional metastases. FOR CITATION: Astashov V.V., Lomshakov A.A., Tsekhmistrenko T.A. et al. Morphology of regional lymph nodes in experimental prostate carcinogenesis. Russian Medical Inquiry. 2022;6(4):171–176 (in Russ.). DOI: 10.32364/2587-6821-2022-6-4-171-176.
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