俄罗斯联邦西北联邦区沃洛格达地区猪场中隐孢子虫的传播

A. Kryazhev
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摘要

隐孢子虫病是一种普遍存在的寄生虫性人畜共患病,是兽医学和世界范围内的一个重大问题。家畜,特别是仔猪,最容易感染这种疾病,然而,俄罗斯联邦这种动物中隐孢子虫属代表的物种组成至今仍不清楚。在俄罗斯联邦西北部,以沃洛格达地区为例,在采用工业维护技术的养猪场,以及采用最新分子遗传技术的私人养猪场,即利用巢式PCR获得的18S rRNA基因片段的高效扩增文库测序,我们首次在所有年龄组的被检测动物中检测到scrofarum的寄生。总共研究了400只动物的样本,即在工业养猪场饲养的150只动物(每个年龄组30只)和在农场饲养的250只动物(每个年龄组50只)。猪场和农场的感染率分别为34.0%(51/150)和32.4%(81/250)。13-24周龄育肥的动物最容易感染。
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SPREAD OF CRYPTOSPORIDIUM SCROFARUM ON PIG FARMS OF THE VOLOGDA REGION OF THE NORTH-WESTERN FEDERAL DISTRICT OF THE RUSSIAN FEDERATION
Cryptosporidiosis is an ubiquitous parasitic zoonosis that is a significant problem in veterinary medicine and medicine worldwide. Livestock animals, in particular piglets, are most often susceptible to this disease, however, the species composition of representatives of the genus Cryptosporidium in this species of animals in the Russian Federation has remained unknown to this day. For the first time in the Russian Federation, in the north-west, by the example of the Vologda Region, on pig farms with industrial maintenance technology, as well as on private farms using the latest molecular genetic techniques, namely using highly-efficient sequencing of amplicon libraries of 18S rRNA gene fragments obtained as a result of a nested PCR, we detected parasitism of C. scrofarum in all age groups of examined animals. In total, samples from 400 animals were studied, namely 150 animals kept in industrial pig complexes (30 in each age group) and 250 (50 in each age group) animals kept on farms. The infection rate in the animals kept on pig farms was 34.0% (51/150), and 32.4% (81/250) on farms. Animals that are fattened at the age of 13-24 weeks were most susceptible to infection.
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