菲律宾民族植物下调与铜绿假单胞菌群体感应介导的生物膜形成相关的激光表达

R. Santos, Wilson R. Jacinto, Khristina G. Judan Cruz
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引用次数: 2

摘要

抗菌药物耐药性的普遍存在带来了开发控制传染病的新方法的激增。以群体感应(QS)控制的细菌毒力因子为靶点是一种很有前途的抗致病性药物策略。菲律宾龙舌兰-龙舌兰民族植物的提取物:牙买加石竹、腺花、薇甘菊、水蜜桃、臭椿、冬葵、乌龙花、菲律宾龙舌兰、水子叶、番泻草、龙舌兰、五子叶、榕树、龙舌兰、龙舌兰采用微滴板生物膜形成试验,筛选铜绿假单胞菌临床分离物和铜绿假单胞菌PNCM 1335中esculentium和Talahib(学名不详)对生物膜形成的QS抑制活性。薇甘菊、紫堇花、普通花、中间花、籼稻叶、豆科植物和塔拉希布提取物对铜绿假单胞菌临床分离物生物膜的形成有抑制作用。牙买加菊提取物的生物膜形成明显减少。、薇甘菊、水仙、凡夫花、野花、五角草、中间花、印度花、豆科花和芥蓝。对铜绿假单胞菌pncm1335的抗性。通过绝对定量的qRT-PCR分析,薇甘菊、Talahib和A. intermedia的RNA提取物在两种测试菌中均表现出lasR的下调。本文介绍了这些民族植物药在抑制细菌毒力、避免抗生素耐药性方面的应用前景。
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PHILIPPINE ETHNOBOTANICALS DOWNREGULATE lasR EXPRESSION LINKED TO QUORUM SENSING-MEDIATED BIOFILM FORMATION IN Pseudomonas aeruginosa
The prevalence of antibacterial resistance has brought about a growing surge to develop novel approaches to control infectious diseases. Targeting Quorum-sensing (QS) - controlled virulence factors in bacteria has indicated a promising strategy for antipathogenic drugs. Extracts of Philippine Ilongot-Egongot ethnobotanicals Stachytarpeta jamaicensis, Adenanthera intermedia, Mikania micrantha, Hyptis suaveolens, Premna odorata, Cymbopogan winterianus, Phyllanthus urinaria, Dillenia philippinensis, Hydrocotyle vulgaris, Senna alata, Urena lobata, Ceiba pentandra, Ficus sp., Eleusine indica, Diplazium esculentum and Talahib (no known scientific name) were screened using microtiter plate biofilm formation assay for their QS inhibition activity against biofilm formation in Pseudomonas aeruginosa clinical isolate and Pseudomonas aeruginosa PNCM 1335. Extracts of M. micrantha, H. suaveolens flowers, H. vulgaris, A. intermedia, E. indica leaves, D. esculentum and Talahib have inhibitory effect on P. aeruginosa clinical isolate biofilm formation. Decrease in biofilm formation was shown in extracts of S. jamaicensis., M. micrantha, H. suaveolens, H. vulgaris, U. lobata, C. pentandra, A. intermedia, E. indica, D. esculentum and Talahib. against P. aeruginosa PNCM 1335.  RNA extracts of M. micrantha, Talahib and A. intermedia exhibited downregulation of lasR in both test bacteria using qRT-PCR analysis through absolute quantification. The prospects of these ethnobotanicals to inhibit bacterial virulence avoiding antibiotic resistance is presented in this paper.
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