芽孢Septoria Nodorum在培养和发病过程中产生的多糖降解酶

Plant Science Letters Pub Date : 1984-11-01 DOI:10.1016/0304-4211(84)90204-9

Paolo Magro

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引用次数: 17

摘要

在添加小麦细胞壁的矿物培养基和接种的小麦叶片中，芽孢杆菌均产生细胞壁降解酶(CWDE)(聚半乳糖醛酸酶(PG)、木聚糖酶(XY)和纤维素酶(CX))。等电聚焦分辨率显示PG (pI 6.2和9.4)和XY (pI 5.2)的体内外同工酶模式相同。CX存在于培养滤液中，有一个分辨率较差的峰(pI 4.3-4.7)。在感染组织中，在pI 7.8处出现了额外的峰值。并与其他叶斑病病原菌的一些特性进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Production of polysaccharide-degrading enzymes by Septoria Nodorum in culture and during pathogenesis

Septoria nodorum produced cell wall-degrading enzymes (CWDE) (oolygalacturonase (PG), xylanase (XY) and cellulase (CX)) both in mineral medium supplemented with wheat cell walls and in inoculated wheat leaves. The same in vitro and in vivo isoenzyme pattern for PG (pI 6.2 and 9.4) and XY (pI 5.2) was exhibited by isoelectric focusing resolution. CX was present in culture filtrates with one poorly resolved peak (pI 4.3–4.7). In infected tissues, an additional peak at pI 7.8 appeared. Some characteristics of S. nodorum polysaccharidases were compared with those of other leaf spot pathogens.

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