螺旋藻提取物对实验大鼠离体肝组织中砷的去除能力

Andalib Mustafa Iqbal Ira, S. Jesmin, S. Akhter, Mahfuza Mazeda Rowshan, E. O. Eva, M. Misbahuddin
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引用次数: 0

摘要

背景:螺旋藻对人体的代谢有多种作用。目的:观察螺旋藻提取物对大鼠离体肝组织中砷的去除作用。方法:本动物研究采用体重160 ~ 200 g的挪威朗埃文斯成年健康雄性大鼠的离体肝组织进行。大鼠为3 ~ 6月龄,2004 ~ 2005年在孟加拉国达卡班班杜谢赫穆吉布医科大学动物舍饲养。测量和所有任务都以非常谨慎的方式执行。采用氢化物发生原子吸收分光光度计测定砷的含量。取一组刚洗过的试管。所有试管均含有2ml酪氨酸溶液和20小块肝组织,保持00C。1 .其余试管均添加2.5 μg/ml三氧化二砷。它们都在370摄氏度的温度下孵育45分钟。然后把纸巾洗干净。螺旋藻提取物以20 μl/ml的剂量加入。第二次孵育也是在370℃下再孵育45分钟。所有的试管都是重复的。结果:观察了不同螺旋藻提取物(E1、E2、E4、E7)对含砷组织中砷的去除效果。空白中砷积累量(平均±se)为6.04±3.05 μg/g蛋白质。两组孵育均给药2.5 μg/ml三氧化二砷后,累积砷量为245.02±22.37 μg/g蛋白质。从标准品中扣除空白,以238.96±19.32 μg / g蛋白作为对照。含砷组织与螺旋藻E1、E2、E4、E7不同提取液在370C下二次孵育45 min,每次提取液浓度为20 μl / ml,经E1提取液二次孵育后,累积砷量为136.40±14.23 μg / g,砷去除率为14.81%。螺旋藻E2萃取物(20 μl/ml)对含砷(2.5 μg/ml)的组织二次孵育,砷含量为242.56±16.50 μg/g(平均±se)。砷的去除率为12.59%。结论:螺旋藻提取物对实验大鼠离体肝组织有明显的除砷作用。孟加拉国国家神经科学研究所学报,2020;6(2):105-109
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Ability of Extracts of Spirulina for the Removal of Arsenic from Isolated Liver Tissues of Experimental Rat
Background: Spirulina has several effects in the metabolism of the body. Objective: The purpose of the present study was to see the ability of extracts of spirulina for the removal of arsenic from isolated liver tissues of rat. Methodology: This animal study was carried out on isolated liver tissues of Long Evans Norwegian adult healthy male rats weighing 160 to 200 g. The rats were 3 to 6 months of age obtained from animal house of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh from 2004 to 2005. Measurements and all tasks were performed in a very careful manner. Atomic Absorption Spectrophotometer with Hydride Generator was used to measure the arsenic level. A set of freshly washed test tubes was taken. All test tubes contained 2ml tyrode solution and twenty small pieces of liver tissue maintaining 00C. No arsenic was added in test tube I. 2.5 μg/ml arsenic trioxide was added rest of the test tubes. They all were incubated for 45 minutes at 370C. Then the tissues were washed properly. The extracts of spirulina was added at 20 μl/ml dose. Second incubation was also for another 45 minutes at 370C. There were duplicates of all test tubes. Result: The effects of different extracts of spirulina (E1, E2, E4 and E7) on the removal of arsenic from arsenic loaded tissue were recorded. Amount of accumulated arsenic (mean ± se) in blank was 6.04 ± 3.05 μg/g of protein. After administration of 2.5 μg/ml arsenic trioxide in both incubation, the amount of accumulated arsenic was 245.02 ± 22.37 μg/g of protein. Blank was deducted from the standard and the value was considered as control and it was 238.96 ± 19.32 μg / g of protein. The arsenic loaded tissues were incubated with different extracts (E1, E2, E4 and E7) of spirulina in second incubation for another 45 minutes at 370C and each extract was for 20 μl / ml. After E1 extract of spirulina in second incubation, amount of accumulated arsenic was 136.40 ± 14.23 μg / g. There was 14.81% of removal of arsenic. Second incubation with E2 extract of spirulina (20 μl/ml) on arsenic loaded (2.5 μg/ml) tissue showed the amount of arsenic 242.56 ± 16.50 μg/g of protein (mean ± se). There was 12.59% of removal of arsenic. Conclusion: In conclusion extracts of spirulina has a significant ability to remove arsenic from isolated liver tissues of experimental rat. Journal of National Institute of Neurosciences Bangladesh, 2020;6(2): 105-109
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