Ecofriendly Spectrophotometric Methods for the Simultaneous Determination of Flupirtine Maleate and Thiocolchicoside: Evaluation of Greenness Profile

Abstract The present study represents four simple UV spectrophotometric methods that are accurate, precise, and ecofriendly for estimating flupirtine maleate and thiocolchicoside in pharmaceutical formulation. The four methods are the second order derivative of zero order spectra, the first derivative of ratio spectra, the extended ratio subtraction technique, and the absorption factor method. The proposed methods have linear calibration curves in the range of 14-26 to 2.80-5.2 µg mL-1 for flupirtine maleate and thiocolchicoside, correspondingly. The proposed methods are evaluated toward synthetic mixtures of the drugs as mentioned earlier and then extended to commercial pharmaceutical formulations of the analytes of interest. The findings of the assay for determining the cited drugs in pharmaceutical tablets were statistically analysed with those of the published HPLC method, implying that no discernible difference exists between the suggested and published procedures. The analytical Eco scale, Agree metrics, and Green analytical procedure index was used to assess the method's greenness scores. The methods have been validated according to ICH criteria and confirmed to be within acceptable limits. GRAPHICAL ABSTRACT