An in Vitro Study of the Effects of Different Dentin Bonding Agents on the Prevention of Tooth Discoloration and the Sealing Ability of Calcium Silicate-Based Cement in Regenerative Endodontic Procedures

This study aimed to compare the effects of applying different types of dentin bonding agents (DBAs) on tooth and on the sealing ability of CSC in regenerative endodontic procedures (REPs). Specimens were made from 120 human first premolars and categorized into the following groups based on the presence and the type of DBA: Group I, no bonding; Group II, One-Step; Group III, Clearfil SE Bond; and Group IV, Single Bond Universal. Subsequently, the groups were categorized into Subgroup a and Subgroup b, in which Endocem MTA® Premixed Regular and Biodentine were used, respectively. The CIE L*a*b* on the photos taken prior to TAP placement (S0), 1 week after TAP placement (S1), 2 weeks after TAP placement (S2), 4 weeks after TAP placement (S3) and 2 weeks after CSC placement (S4) was used to assess discoloration. The samples were submerged in a 2% methylene blue solution and magnified by 30 times under a stereomicroscope to assess microleakage. The application of DBA during REP reduced crown discoloration, while Biodentine showed minimal discoloration with or without DBA and regardless of the DBA type. Additionally, the application of DBA did not increase microleakage.