Asal Ebrahimzadeh, Elnaz Khanalizadeh, Shahla Khodabakhshaghdam, D. Kazemi, Ali Baradar Khoshfetrat
{"title":"明胶改性对酶凝胶性果胶-明胶水凝胶性能的影响","authors":"Asal Ebrahimzadeh, Elnaz Khanalizadeh, Shahla Khodabakhshaghdam, D. Kazemi, Ali Baradar Khoshfetrat","doi":"10.1177/08839115221119210","DOIUrl":null,"url":null,"abstract":"Injectable in situ-forming hydrogels appears to be a promising approach for tissue engineering applications. In this study, the effect of phenol moiety (Ph) addition to gelatin in enzymatically-gellable modified pectin hydrogel (Pec-Ph) was studied. Addition of gelatin-Ph to Pec-Ph (Pec-Ph/Gel-Ph) altered the physical properties of Pec-Ph-based hydrogels as compared to unmodified gelatin (Pec-Ph/Gel) addition. Swelling ratio and degradation rates of the Pec-Ph/Gel-Ph hydrogel decreased 35% and 50%, respectively, and the elasticity of Pec-Ph/Gel-Ph hydrogel was higher than the Pec-Ph/Gel hydrogels. Scanning electron microscopy images showed that the existence of phenolic groups in gelatin decreased the pore size of Pec-Ph/Gel-Ph hydrogels. Culture of chondrocyte cells in the Pec-Ph/Gel-Ph hydrogels showed more metabolic activity (4×) during a 14-day culture period. Hydrogels subcutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. The work showed the potential of Pec-Ph/Gel-Ph hydrogels as a promising in situ injectable hydrogel for soft tissue engineering applications.","PeriodicalId":15038,"journal":{"name":"Journal of Bioactive and Compatible Polymers","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2022-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of gelatin modification on enzymatically-gellable pectin-gelatin hydrogel properties for soft tissue engineering applications\",\"authors\":\"Asal Ebrahimzadeh, Elnaz Khanalizadeh, Shahla Khodabakhshaghdam, D. Kazemi, Ali Baradar Khoshfetrat\",\"doi\":\"10.1177/08839115221119210\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Injectable in situ-forming hydrogels appears to be a promising approach for tissue engineering applications. In this study, the effect of phenol moiety (Ph) addition to gelatin in enzymatically-gellable modified pectin hydrogel (Pec-Ph) was studied. Addition of gelatin-Ph to Pec-Ph (Pec-Ph/Gel-Ph) altered the physical properties of Pec-Ph-based hydrogels as compared to unmodified gelatin (Pec-Ph/Gel) addition. Swelling ratio and degradation rates of the Pec-Ph/Gel-Ph hydrogel decreased 35% and 50%, respectively, and the elasticity of Pec-Ph/Gel-Ph hydrogel was higher than the Pec-Ph/Gel hydrogels. Scanning electron microscopy images showed that the existence of phenolic groups in gelatin decreased the pore size of Pec-Ph/Gel-Ph hydrogels. Culture of chondrocyte cells in the Pec-Ph/Gel-Ph hydrogels showed more metabolic activity (4×) during a 14-day culture period. Hydrogels subcutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. The work showed the potential of Pec-Ph/Gel-Ph hydrogels as a promising in situ injectable hydrogel for soft tissue engineering applications.\",\"PeriodicalId\":15038,\"journal\":{\"name\":\"Journal of Bioactive and Compatible Polymers\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2022-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Bioactive and Compatible Polymers\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1177/08839115221119210\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bioactive and Compatible Polymers","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/08839115221119210","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Influence of gelatin modification on enzymatically-gellable pectin-gelatin hydrogel properties for soft tissue engineering applications
Injectable in situ-forming hydrogels appears to be a promising approach for tissue engineering applications. In this study, the effect of phenol moiety (Ph) addition to gelatin in enzymatically-gellable modified pectin hydrogel (Pec-Ph) was studied. Addition of gelatin-Ph to Pec-Ph (Pec-Ph/Gel-Ph) altered the physical properties of Pec-Ph-based hydrogels as compared to unmodified gelatin (Pec-Ph/Gel) addition. Swelling ratio and degradation rates of the Pec-Ph/Gel-Ph hydrogel decreased 35% and 50%, respectively, and the elasticity of Pec-Ph/Gel-Ph hydrogel was higher than the Pec-Ph/Gel hydrogels. Scanning electron microscopy images showed that the existence of phenolic groups in gelatin decreased the pore size of Pec-Ph/Gel-Ph hydrogels. Culture of chondrocyte cells in the Pec-Ph/Gel-Ph hydrogels showed more metabolic activity (4×) during a 14-day culture period. Hydrogels subcutaneously implanted in rats could also be identified readily without complete absorption and signs of toxicity or any untoward reactions after 1 month. The work showed the potential of Pec-Ph/Gel-Ph hydrogels as a promising in situ injectable hydrogel for soft tissue engineering applications.
期刊介绍：
The use and importance of biomedical polymers, especially in pharmacology, is growing rapidly. The Journal of Bioactive and Compatible Polymers is a fully peer-reviewed scholarly journal that provides biomedical polymer scientists and researchers with new information on important advances in this field. Examples of specific areas of interest to the journal include: polymeric drugs and drug design; polymeric functionalization and structures related to biological activity or compatibility; natural polymer modification to achieve specific biological activity or compatibility; enzyme modelling by polymers; membranes for biological use; liposome stabilization and cell modeling. This journal is a member of the Committee on Publication Ethics (COPE).
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