富血小板血浆20%与自体血清20%实验性角膜碱烧伤炎症反应的比较

Amelia Amelia Safitri Ramadhani
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摘要

目的比较富血小板血浆20%与自体血清20%对氢氧化钠暴露后角膜的炎症反应。方法采用两组后测设计的真实验研究。试验选用18只家兔,分为两组。两组静脉采血后,离心稀释分别获得富血小板血浆20%和自体血清20%。取直径7.0 mm的圆形滤纸,用1N NaOH浸泡60 s,对每只兔右眼中央角膜进行碱烧伤。连续7天每天观察炎症的临床结果。第7天,收集角膜组织进行组织病理学检查,评估角膜中央浸润的PMN中性粒细胞的数量。对所有数据进行统计学分析,以确定研究组之间的差异。结果统计学分析显示,第3天至第7天结膜充血的临床炎症特征有统计学差异,PRP 20%组结膜充血分级低于AS 20%组。其他临床表现,角膜混浊和上皮缺损,两组间无统计学差异。组织病理学检查显示,PRP组角膜中央的PMN中性粒细胞浸润量低于AS组。结论富血小板血浆20%滴眼液可作为角膜碱性损伤急性期的辅助治疗方法之一,对角膜碱性损伤急性期的炎症反应有较好的控制作用。
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COMPARISON OF INFLAMMATORY RESPONSE BETWEEN PLATELET RICH PLASMA 20% AND AUTOLOGUS SERUM 20% IN EXPERIMENTALLY-INDUCED CORNEAL ALKALI BURN
Objective to compare the inflammatory response between platelet rich plasma 20% and autologous serum 20% on cornea after sodium hydroxide exposure. Methods a true experimental study with two groups post-test design. A total of 18 rabbits were used in this study and allocated into two groups. After collecting intravenous blood samples from both group, platelet rich plasma 20% and autologous serum 20%, respectively, were obtained by centrifugation and dilution. Alkali burns were inflicted on the central cornea of each rabbit’s right eye by applying a round filter paper, 7.0 mm in diameter, soaked in 1N NaOH for 60 s. Clinical outcome of the inflammation were observed everyday for seven days. On the seventh day, corneal tissue was collected for histopathological examination to evaluate amount of PMN neutrophils that infiltrate the central cornea. All data were statistically analyzed for difference between the study groups. Resuls statistical analysis shows statistically difference in clinical inflammatory feature of conjunctival hyperemia at day 3 until day 7, in which the group that received PRP 20% got lower conjunctival hyperemia grading compared to the group that received AS 20%. Other clinical features, corneal opacity and epithelial defect, shows no statistically difference between two groups. Histopathological examination shows lower amount of PMN neutrophil infiltration to the central cornea on PRP group, compared to AS group. Conclusio Platelet-rich plasma 20% eyedrop can be used as one of adjuvant therapies and has better control of inflammatory response towards alkaline injury of the cornea during acute phase.
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