基于纳米金修饰电极的葡萄糖传感器的制备

Rong Zou, L. Ling, Lin Wang
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摘要

近年来,纳米材料因其具有粒径小、比表面积大、表面反应活性高、催化活性好、吸附能力强等优点而备受关注。本文采用自组装技术制备了基于纳米金的葡萄糖无酶生物传感器。首先,按以下步骤制备金胶体:用细绳将100ml 0.01%的HAuCl4水溶液加热至沸腾,然后快速加入2ml 1%的柠檬酸三钠,煮沸后,细绳保持15min,混合物的颜色由淡黄色变为蓝色,最后达到红宝石红色,然后将仔细清洗过的Ag电极浸入金胶体中,得到无酶葡萄糖传感器。结果表明,在最佳条件下,该传感器对葡萄糖具有良好的电流响应,线性范围为0.2 ~ 175.2μmol/L,检出限为0.15μmol/L。此外,所制备的生物传感器具有优异的稳定性、高灵敏度和良好的选择性。
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Preparation of the glucose sensor based on nano-au modified electrode
In recent years, the nano-materials, which have advantages of small particle size, large specific surface area, high surface reactive activity, good catalytic activity and strong adsorptivity, have attracted considerable attention. In this paper, the glucose non-enzymatic biosensor based on nano-Au has been fabricated by self-assembly technology. Firstly, the gold colloid were prepared according to the follow steps: With string, 100 ml of 0.01 % HAuCl4 aqueous solution was heated to a boiling condition, then 2 ml of 1 % trisodium citrate was rapidly added After boiling, keeping string for 15 min, the color of the mixture changed from pale yellow to blue, and finally arrived at ruby-red Then a carefully cleaned Ag electrode was dipped into the gold colloid to obtain the non-enzymatic glucose sensor. Their electrochemical properties were investigated The sensos have been successfully applied for the determination of glucose and the result also showed that the non-enzymatic glucose sensor had good current response to glucose in alkaline medium Under optimal conditions, the developed glucose biosensor exhibited fast response to glucose and linear ranges were0.2 to 175.2μmol/L, with a detection limit of 0.15μmol/L. In addition, the as-prepared biosensor exhibiter superior stability high sensitivity and good selectivity.
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