耻垢分枝杆菌用d -木糖糖生产木糖醇的研究

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI:10.1016/0385-6380(88)90126-4

Ken Izumori, Keiji Tuzaki

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引用次数: 47

摘要

耻垢分枝杆菌在好氧和厌氧条件下将d -木糖糖转化为木糖醇。在含木糖醇或d -甘露醇培养基上生长的细胞，在厌氧条件下(N2)木糖醇的产率可达70%左右。用海藻酸钙固定的细胞与水洗细胞的木糖醇生产活性几乎相同。利用凝固芽孢杆菌固定化d -木糖异构酶和耻垢分枝杆菌固定化细胞，以d -木糖为原料制备木糖醇。10 g d -木糖生成4 g木糖醇，反应混合物中保留5 g d -木糖;未检测到d -木酮糖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Production of xylitol from D-xylulose by Mycobacterium smegmatis

Mycobacterium smegmatis transformed D-xylulose to xylitol in washed cell reactions under aerobic and anaerobic conditions. The yield of xylitol reached about 70% in anaerobic conditions (in N₂) by cells grown on media containing xylitol or D-mannitol. Cells immobilized with Ca-alginate had almost the same activity of xylitol production as washed cells.

Xylitol was produced from D-xylose using commercial immobilized D-xylose isomerase from Bacillus coagulans and immobilized cells of M. smegmatis. From 10 g of D-xylose, 4 g of xylitol was produced and 5 g of D-xylose remained in the reaction mixture; no D-xylulose was detected.

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