Evaluation of anti-inflammatory activity of methanol extract of Barleria Cristata leaves by in vivo and in vitro methods

Evaluation anti-inflammatory Barleria by in Abstract The methanol extract of Barleria Cristata leaves (BCM) was evaluated for anti-inflammatory activity using in vivo and in vitro methods. In the in vivo inflammation tests, BCM significantly inhibited edema produced by histamine and serotonin in rats, also reduces significantly acetic acid-induced vascular permeability in mice dose dependently. In the in vitro tests, the probable supporting mode by which BCM mediates its effects on inflammatory conditions was studied on red blood cells (RBC’s) exposed to hypotonic solution and thermally induced protein denaturation. BCM exhibited significant membrane-stabilizing property. Thermal induced protein denaturation was significantly inhibited by the extract. The effect was compared with the activity of indomethacin and cyproheptadine as reference standard against different types of inflammation. Results of the study revealed that BCM possesses significant anti-inflammatory activity.