农杆菌与大豆甘氨酸max (L.)的相互作用组织培养中的叶片外植体

D. T. Kudirka, S. Colburn, M. Hinchee, M. Wright
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引用次数: 31

摘要

大豆叶柄表面损伤(Glycine max (L.))接种致瘤性农杆菌菌株后,“北京”的Merr.)叶片形成肿瘤生长,对opines检测呈阳性。在接种48 h期间,未接种的外植体和接种了A136(无毒)、A208(致瘤性)和ASE1-200(非致瘤性)菌株的外植体在创面出现了有丝分裂活性,符合经典的创面反应描述;然而,外植体转移到组织培养基后不久,接种了致瘤芽孢杆菌的外植体的形态发生模式与未接种和接种A136的对照不同。这种发育偏差与细菌中Ti质粒的存在有关。非致瘤芽胞杆菌接种的外植体表现出介于对照和致瘤菌接种的外植体之间的形态发生模式。这表明后者……
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Interactions of Agrobacterium tumefaciens with soybean (Glycine max (L.) Merr.) leaf explants in tissue culture
Wounded petiole surfaces of excised soybean (Glycine max (L.) Merr.) leaves of 'Peking' formed neoplastic growths testing positive for opines after inoculation with a tumorigenic strain of Agrobacterium tumefaciens. During the 48-h inoculation period, uninoculated explants and explants inoculated with A136 (avirulent), A208 (tumorigenic), and ASE1-200 (nontumorigenic) strains of A. tumefaciens showed initiation of mitotic activity at the wound surface conforming with classical descriptions of the wound response; however, shortly after explant transfer to tissue culture medium, morphogenetic patterns in explants inoculated with tumorigenic A. tumefaciens deviated from the uninoculated and A136 inoculated controls. This developmental deviation was correlated to the presence of the Ti plasmid in the bacterium. Explants inoculated with nontumorigenic A. tumefaciens showed a morphogenetic pattern intermediate between the controls and explants inoculated with tumorigenic bacteria. This suggests that the latter ...
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