Designing of putative siRNA to inhibit dengue virus replication

http://dx.doi.org/10.21276/IJRDPL.22780238.2018.7(5).3115-3118 ABSTRACT: Dengue virus is mosquito-borne virus that manifests itself in human infections with dengue fever (DF) to dengue hemorrhagic fever (DHF). DHF can lead to development of dengue shock syndrome (DSS). RNAi works by silencing the target gene expression using siRNA. Hence there arises an urgent need to design potential siRNA against the target sequence of dengue virus gene to control its replication and pathogenicity. This study is aimed to predict self-potential designed siRNA-based therapeutics that might be used for the treatment of dengue virus infection. The prediction of potential siRNA was done by using various computational tools as searching target sequences, multiple sequences alignment, secondary structure prediction, siRNA-target sequence interaction prediction and finally the evaluation of effectiveness of predicted siRNA. Ten pair of potential siRNAs were predicted and designed rationally for silencing five target genes (Capsid, CprM, NS1, NS3 and NS5) of dengue virus used in the study through RNAi technology. The outcomes of this study are ten pair of potential siRNA molecules which might be used as a potential antiviral RNA based therapeutics to suppress the dengue virus replication.