OsHox-6基因的过表达增加了水稻分蘖数,但造成了产量损失

S. Rahmawati, Chairunnisa Chairunnisa, E. Erdayani, S. Nugroho, A. Estiati
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摘要

OsHox-6属于转录因子同源结构域亮氨酸拉链(HD-Zip)蛋白亚家族I,功能未知。本研究在水稻组成启动子OsLEA3的控制下,对含有OsHox-6基因额外拷贝的两个纯合子转基因水稻品系(S29-62-2和S.40.4-158-1)的表型进行了表征,并评价了它们对水分胁迫的耐受性。实时荧光定量PCR (real-time quantitative PCR, qRT-PCR)结果显示,与野生型对照相比,OsHox-6基因在正常灌溉条件下表达量增加了5-10倍,在水分胁迫条件下表达量增加了10-20倍。过表达OsHox-6的转基因植株在正常灌溉条件下表现出表型改变,诱导分蘖形成,表明顶端优势降低。转基因植株的总粒数显著增加,空粒数也显著增加(~16-22%)。在施加水分胁迫后,转基因品系的空粒数更高(平均高达83%)。此外,对水分损失率、相对含水量和抗旱性指数(DRI)的观察表明,过表达OsHox-6对水分胁迫的耐受性没有显著提高。需要进一步的研究来揭示OsHox-6对水和其他非生物胁迫响应的详细机制。
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Overexpression of OsHox-6 Gene Enhanced Tiller Number in Rice But Induced Yield Penalty
OsHox-6, belongs to the transcription factor homeodomain leucine zipper (HD-Zip) protein sub-family I, has unknown function. This study was aimed to characterize the phenotypes of two homozygous transgenic rice lines (S29-62-2 and S.40.4-158-1) containing an extra copy of OsHox-6 gene under the control of a rice constitutive promoter, OsLEA3, and to evaluate their tolerance to water stress. A real-time quantitative PCR (qRT-PCR) showed that the transcript expression of OsHox-6 gene in the transgenic lines increased 5-10 folds under a normal irrigation and 10-20 folds after exposure to water stress conditions as compared to its wild type control. Transgenic plants overexpressing OsHox-6 exhibited phenotypic alteration at the normal irrigation by inducing tiller formation, suggesting a decrease in the apical dominance. Transgenic plants also showed significant enhancement in the total grain number, however, the number of empty grains  also increased significantly (~16-22%).  After imposed to the water stress, the number of empty grains in the transgenic lines was even higher (up to 83% in average). Furthermore, observations on the water loss rates, relative water contents and drought resistance indices (DRI) suggested that the overexpression of OsHox-6 did not significantly increase tolerance to water stress.  Further research is required to reveal the detailed mechanisms of OsHox-6 in response to water and other abiotic stresses.
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