{"title":"反相高效液相色谱法同时测定USP溶出介质和模拟胃液中利福平、异烟肼和吡嗪酰胺的含量","authors":"T. Mariappan, Baljinder Singh, Saranjit Singh","doi":"10.1211/146080800128736187","DOIUrl":null,"url":null,"abstract":"A liquid-chromatographic reversed-phase method employing a C18 column has been developed for simultaneous analysis of rifampicin, isoniazid and pyrazinamide in 0.1 M hydrochloric acid dissolution medium and in simulated gastric fluid. Good separations were achieved by employing a gradient prepared from methanol and 0.01M sodium dihydrogen orthophosphate buffer containing 0.05% w/v tetramethylammonium chloride (pH adjusted to 3.5). The detection wavelength was 254 nm. The method was validated for linearity, accuracy, precision and ruggedness. It was found to work well for analysis of the three drugs in the presence of degradation products and even for samples containing ethambutol, which does not interfere because of lack of UV absorption.","PeriodicalId":19946,"journal":{"name":"Pharmacy and Pharmacology Communications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"18","resultStr":"{\"title\":\"A Validated Reversed‐Phase (C18) HPLC Method for Simultaneous Determination of Rifampicin, Isoniazid and Pyrazinamide in USP Dissolution Medium and Simulated Gastric Fluid\",\"authors\":\"T. Mariappan, Baljinder Singh, Saranjit Singh\",\"doi\":\"10.1211/146080800128736187\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A liquid-chromatographic reversed-phase method employing a C18 column has been developed for simultaneous analysis of rifampicin, isoniazid and pyrazinamide in 0.1 M hydrochloric acid dissolution medium and in simulated gastric fluid. Good separations were achieved by employing a gradient prepared from methanol and 0.01M sodium dihydrogen orthophosphate buffer containing 0.05% w/v tetramethylammonium chloride (pH adjusted to 3.5). The detection wavelength was 254 nm. The method was validated for linearity, accuracy, precision and ruggedness. It was found to work well for analysis of the three drugs in the presence of degradation products and even for samples containing ethambutol, which does not interfere because of lack of UV absorption.\",\"PeriodicalId\":19946,\"journal\":{\"name\":\"Pharmacy and Pharmacology Communications\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"18\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacy and Pharmacology Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1211/146080800128736187\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacy and Pharmacology Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1211/146080800128736187","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A Validated Reversed‐Phase (C18) HPLC Method for Simultaneous Determination of Rifampicin, Isoniazid and Pyrazinamide in USP Dissolution Medium and Simulated Gastric Fluid
A liquid-chromatographic reversed-phase method employing a C18 column has been developed for simultaneous analysis of rifampicin, isoniazid and pyrazinamide in 0.1 M hydrochloric acid dissolution medium and in simulated gastric fluid. Good separations were achieved by employing a gradient prepared from methanol and 0.01M sodium dihydrogen orthophosphate buffer containing 0.05% w/v tetramethylammonium chloride (pH adjusted to 3.5). The detection wavelength was 254 nm. The method was validated for linearity, accuracy, precision and ruggedness. It was found to work well for analysis of the three drugs in the presence of degradation products and even for samples containing ethambutol, which does not interfere because of lack of UV absorption.
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