{"title":"菝葜根茎中β -谷甾醇的分离鉴定及其抗氧化活性评价","authors":"A. Bhat, A. Alia, G. M. Rather, B. Kumar","doi":"10.26438/ijsrbs/v6i2.111118","DOIUrl":null,"url":null,"abstract":"The aim of this study is isolation, identification and characterized the bioactive compounds from the rhizomes of arisaema utile. Preliminary Phytochemical screening of the rhizome extract of arisaema utile revealed the presence of Steroids, terpenoids, Flavonoids, Alkaloids, Saponins & Carbohydrates. The air dried rhizomes were pulverized to powder, subjected to Soxhlet extraction and compound Isolation. The isolated compound was colorless crystalline, which was further subjected to IR, 13 CNMR and 1 HNMR for proper characterization and elucidation of the structure. The compound was concluded as βSitosterol. Antioxidant activity of the isolated compound was measured by DPPH assay under in-vitro condition. The isolated compound showed most promising radical scavenging activity at concentration of 10μg/ml.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"5 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Isolation and Characterisation of Beta-sitosterol from the rhizomes of Arisaema utile and its Evaluation for Antioxidant Activity\",\"authors\":\"A. Bhat, A. Alia, G. M. Rather, B. Kumar\",\"doi\":\"10.26438/ijsrbs/v6i2.111118\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this study is isolation, identification and characterized the bioactive compounds from the rhizomes of arisaema utile. Preliminary Phytochemical screening of the rhizome extract of arisaema utile revealed the presence of Steroids, terpenoids, Flavonoids, Alkaloids, Saponins & Carbohydrates. The air dried rhizomes were pulverized to powder, subjected to Soxhlet extraction and compound Isolation. The isolated compound was colorless crystalline, which was further subjected to IR, 13 CNMR and 1 HNMR for proper characterization and elucidation of the structure. The compound was concluded as βSitosterol. Antioxidant activity of the isolated compound was measured by DPPH assay under in-vitro condition. The isolated compound showed most promising radical scavenging activity at concentration of 10μg/ml.\",\"PeriodicalId\":14378,\"journal\":{\"name\":\"International Journal of Scientific Research in Biological Sciences\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Scientific Research in Biological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.26438/ijsrbs/v6i2.111118\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Scientific Research in Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26438/ijsrbs/v6i2.111118","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation and Characterisation of Beta-sitosterol from the rhizomes of Arisaema utile and its Evaluation for Antioxidant Activity
The aim of this study is isolation, identification and characterized the bioactive compounds from the rhizomes of arisaema utile. Preliminary Phytochemical screening of the rhizome extract of arisaema utile revealed the presence of Steroids, terpenoids, Flavonoids, Alkaloids, Saponins & Carbohydrates. The air dried rhizomes were pulverized to powder, subjected to Soxhlet extraction and compound Isolation. The isolated compound was colorless crystalline, which was further subjected to IR, 13 CNMR and 1 HNMR for proper characterization and elucidation of the structure. The compound was concluded as βSitosterol. Antioxidant activity of the isolated compound was measured by DPPH assay under in-vitro condition. The isolated compound showed most promising radical scavenging activity at concentration of 10μg/ml.
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