泰国传统肉肠“Mhom”的微生物特征

W. Samappito, B. Leenanon, R. Levin
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引用次数: 8

摘要

泰国传统的肉肠,即Mhom,是泰国东北部地区流行的发酵肉制品,但关于Mhom的科学信息却很少。从Chaiyaphum (CP)、Khonkaen (KK)、Maha Sarakham (MK)和Kalasin (KS) 4个省分离到71株乳酸菌。结果表明,Mhom编码CP1、CP2、KK、MK和KS的ph值分别为4.74、4.94、4.77、4.30和4.76,总酸度占乳酸的百分比分别为1.16、0.78、1.56、0.94和0.86。Mhom编码CP1、CP2、KK、MK和KS的乳酸菌总数分别为6.0 × 10.6、7.8 × 10.6、5.4 × 10.6、6.4 × 10.6和1.0 × 10.7 cfu/g。用API 50ch对分离的细菌进行生化检测,鉴定为弯曲乳杆菌、德尔布鲁氏乳杆菌、嗜酸乳杆菌、副干酪乳杆菌、短乳杆菌、戊糖乳杆菌、中间乳杆菌、植物乳杆菌、farciminis乳杆菌、发散肉杆菌、戊糖乳球菌和肠球菌。在姆姆发现的这些乳酸菌菌株可能在成熟过程中发挥重要作用,从而在最终产品中产生独特的感官特征。它们是根据在生态位的普遍条件下最具竞争力的能力从产品微生物群中自然选择出来的。分离和鉴定的乳酸菌可作为姆姆酒生产中的发酵剂,使最终产品的感官质量更加均匀,没有姆姆酒自然发酵中常见的不良风味和微生物,产品也可以安全食用。
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Microbiological Characteristics of ''Mhom'', a Thai Traditional Meat Sausage
A Thai traditional meat sausage, namely Mhom, is a popular fermented meat product in the northeastern region of Thailand and yet little scientific information about Mhom is known. Seventy-one isolates of lactic acid bacteria were isolated from Mhom made in four provinces i.e. Chaiyaphum (CP), Khonkaen (KK), Maha Sarakham (MK), and Kalasin (KS). It was found that pHs of Mhom coded CP1, CP2, KK, MK and KS were 4.74, 4.94, 4.77, 4.30 and 4.76, respec- tively, and % total acidity as lactic acid was 1.16, 0.78, 1.56, 0.94, and 0.86, respectively. In addition, the total lactic acid bacteria in the products were 6.0 x 10 6 , 7.8 x 10 6 , 5.4 x 10 6 , 6.4 x 10 6 and 1.0 x 10 7 cfu/g for Mhom coded CP1, CP2, KK, MK and KS, respectively. The bacterial isolates were then subjected to biochemical tests using the API 50 CH and they were identified as Lactobacillus curvatus, L. delbrueckii, L. acidophilus, L. paracasei, L. brevis, L. pentosus, L. mesenter- oides, L. plantarum, L. farciminis, Carnobacterium divergens, Pediococcus pentosaceus and Enterococcus. These strains of lactic acid bacteria found in Mhom may play significant roles in the ripening process resulting in unique sensory char- acteristics in the end products. They were naturally selected from the product microbiota by the ability to best compete under the prevailing conditions of the ecological niche. The lactic acid bacteria isolated and identified could be used as starters in Mhom production to obtain a more uniform sensory quality in the end products without undesirable flavors and microorganisms that may often be found in a natural fermentation in Mhom and the products could also be consumed safely.
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