Alterations of miR-4772-3pand miR-3173-3p Expression in Tissue Compared to Normal Tissue by Real-time PCR

Background: In recent years, many studies have focused on the dysregulation of miRNA expression in cancer. These structures act as oncogenes and suppressors. In the present study, the expression levels of miR-3173-3p and miR-4772-3p were investigated in the tissue of glioblastoma multiforme (GBM) patients and healthy individuals. Methods: The present study was performed on 50 patients with GBM and 50 healthy individuals as the control group. Real-time PCR technique was used to determine the tissue levels of miR-3173-3p and miR-4772-3p. The biomarker evaluation of miR-3173-3p and miR-4772-3p was assessed using ROC curve analysis. Results: The expression level of miR-4772-3p was higher in women with GBM (P-value = 0.03). Increased concentration of miR-3173-3p in tissue of GBM patients showed a significant correlation with a final grade of disease progression (grades III and IV) (P-value = 0.007). ROC curve analysis and cut-off of 0.6939 showed that miR-4772-3p could detect the presence of malignant GBM with 88.89 sensitivity and 100.0 specificity. Conclusions: The present study reveals the potential oncogenic role of miR-4772-3p. Moreover, the progression of glioblastoma cancer in the final stages can be assessed by measuring the level of miR-4772-3p in patients’ tissue.