霉菌毒素检测的新兴技术

C. Maragos
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引用次数: 59

摘要

检测真菌毒素的分析方法的发展历史是丰富多样的。方法开发遵循了一个有点类似于达尔文进化的过程:方法是根据分析人员最需要的特征来选择的。通常,这导致了准确和敏感的检测方法的发展,反复强调提高检测速度和降低检测成本。就像进化一样,也有激进的发展,增量的发展,以及失宠的技术,只是为了重新发现。这篇综述的重点是真菌毒素检测技术的最新发展,特别强调已经开始出现的各种形式的生物传感器。具体来说,本文描述了倏逝波技术(表面等离子体共振、光纤传感器)、横向流动和试纸装置、荧光偏振和时间分辨荧光、微珠测定和毛细管电泳免疫测定的最新发展。新兴技术面临的挑战是在分析实验室之外的环境中证明其优于更传统、更成熟的技术。
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Emerging Technologies for Mycotoxin Detection
The history of the development of analytical methods for detecting fungal toxins is rich and varied. Method development has followed a process somewhat akin to Darwinian evolution: methods are selected based upon the characteristics most desirable to the analyst. Typically, this has lead to the development of accurate and sensitive methods for their detection, with a recurring emphasis on improving the speed and lowering the costs of the assays. Like evolution, there have been radical developments, incremental developments, and techniques that have fallen from favor only to be rediscovered. This review focuses on recent developments in technologies for detection of mycotoxins, with a particular emphasis on the myriad forms of biosensors that have begun to appear. Specifically, recent development in evanescent wave technologies (surface plasmon resonance, fiber optic sensors), lateral flow and dipstick devices, fluorescence polarization and time‐resolved fluorescence, microbead assays, and capillary electrophoretic immunoassays, are described. The challenge for the emerging technologies is to demonstrate advantages over the more conventional, and better established, techniques in settings outside the analytical laboratory.
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