酵母对油菜黄单胞菌的拮抗作用。大田白菜叶面上的油菜病菌

S. P. Assis, R. Mariano, S. Michereff, Gil Silva, Elizabeth A. A. Maranhão
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引用次数: 22

摘要

从白菜叶面中分离得到20株酵母菌,对其对油菜黄单胞菌的拮抗活性进行了评价。露营者,在野外。白菜属植物。与拮抗剂和病原菌混悬液同时粉碎。10 d后,通过叶损面积百分比对植株进行评价。还计算了疾病严重程度降低百分比(DSR%)。酵母菌分离株LR32、LR42和LR19的DSR分别为72%、75%和79%。这些拮抗剂在7个不同的应用时期进行了与病原体接种相关的试验(T1=4 d;同时T2 =;T3=4 d后;T4=4 d +同时;T5=4 d +同时;T6=4 d前+ 4 d后;T7=4天前+同时+ 4天后)。T7、T4、T5和T6处理中,LR42(71%)、LR42(67%)、LR35(69%)和LR19(68%)的dsr最高,与其他处理差异有统计学意义。同样的酵母拮抗剂也用不同的白菜品种(Fuyutoyo、Master-325、Matsukaze、Midori、Sekai I和Red Winner)进行了防治黑腐病的试验。dsr为58% ~ 61%,品种间差异不显著。
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ANTAGONISM OF YEASTS TO XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ON CABBAGE PHYLLOPLANE IN FIELD
Twenty yeast isolates, obtained from cabbage phylloplane, were evaluated for antagonistic activity against Xanthomonas campestris pv. campestris, in field. Plants of cabbage cv. Midori were pulverized simultaneously with suspensions of antagonists and pathogen. After 10 days, plants were evaluated through percentage of foliar area with lesions. Percentage of disease severity reduction (DSR%) was also calculated. Yeast isolates LR32, LR42 and LR19 showed, respectively, 72, 75 and 79% of DSR. These antagonists were tested in seven different application periods in relation to pathogen inoculation (T1=4 d before; T2=simultaneously; T3=4 d after; T4=4 d before + simultaneously; T5=4 d after + simultaneously; T6=4 d before + 4 d after; T7=4 d before + simultaneously + 4 d after). The highest DSRs were showed by LR42 (71%), LR42 (67%), LR35 (69%) and LR19 (68%) in the treatments T7, T4, T5 and T6, which significantly differed from the others. The same yeast antagonists were also tested for black rot control using different cabbage cultivars (Fuyutoyo, Master-325, Matsukaze, Midori, Sekai I and Red Winner). The DSRs varied from 58 to 61%, and there was no significant difference among cultivars.
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