超声辅助空化对常见乳制品孢子形成菌生物膜形成能力的影响

Q2 Agricultural and Biological Sciences Dairy Science & Technology Pub Date : 2023-01-28 DOI:10.3390/dairy4010007
T. Almalki, S. Anand
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引用次数: 0

摘要

热致产孢菌在热处理后仍能存活,并能在接触食物表面形成生物膜,难以清洁,并可能对奶制品造成交叉污染。据推测,空化会影响孢子形成物附着在接触表面并形成生物膜的能力。将常见的乳制品产孢菌嗜脂热地杆菌、地衣芽孢杆菌和嗜热孢子芽孢杆菌分别接种于无菌脱脂乳中,接种水平为6.0 log CFU/mL。接种后的样品以80%的振幅进行空化处理,每次10分钟。在静态条件下,采用前样和后样在不锈钢板上显影生物膜。扫描电镜观察发育的生物膜。所有实验均为三次重复,采用t检验进行统计学分析。三种产孢菌的平均加样量分别为7.2、8.0和7.7 log CFU/mL。空化后计数分别显著降低至3.4、4.2和3.7 log CFU/mL。空化前72 h 3种孢子菌的生物膜计数分别为5.35、6.42和6.5 log CFU/cm2,空化后3种孢子菌的生物膜计数分别为4.39、5.44和4.39 log CFU/cm2,显著降低(p < 0.05)。结果表明,空化后嗜热G. stearothermophilus形成的生物膜最少。虽然超声波处理减少了孢子形成细菌的数量,但幸存者仍然保留了附着在不锈钢食物接触面上的能力。
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Ultrasound-Assisted Cavitation Effect on the Biofilm-Forming Ability of Common Dairy Sporeformers
Thermoduric sporeformers survive heat treatment and can form biofilm on contact food surfaces that is difficult to clean and may cause cross contamination to milk products. It was hypothesized that cavitation would influence sporeformers’ ability to attach to contact surfaces and form biofilm. Common dairy sporeformers of Geobacillus stearothermophilus, Bacillus licheniformis, and Bacillus sporothermodurans were individually inoculated in sterile skim milk at the levels of 6.0 log CFU/mL. Inoculated samples were treated by cavitation at 80% amplitudes for 10 min each. Pre and post samples were used to develop biofilms on stainless steel coupons under static conditions. Scanning electron micrograph was used to observe the developed biofilms. All the experiments were conducted in triplicate and were statistically analyzed using a t test. The average counts of spiked milk samples were 7.2, 8.0, and 7.7 logs CFU/mL, respectively, for the three sporeformers. Post-cavitation counts were reduced significantly to 3.4, 4.2, and 3.7 logs CFU/mL, respectively. Pre-cavitation biofilm counts of the three sporeformers were 5.35, 6.42, and 6.5 logs CFU/ cm2, respectively in 72 h. The three sporeformers’ biofilm showed significantly (p < 0.05) lower counts after cavitation of 4.39, 5.44, and lower counts of 4.39 logs CFU/cm2, respectively, for the three organisms. The result showed that G. stearothermophilus formed the least biofilms among others after cavitation. Although the ultrasonication treatment reduced the number of sporeformer bacteria, the survivors still retained the ability to attach to the stainless-steel food contact surfaces.
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来源期刊
Dairy Science & Technology
Dairy Science & Technology 农林科学-食品科技
CiteScore
2.30
自引率
0.00%
发文量
0
审稿时长
2 months
期刊介绍: Information not localized
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