Diazotizasyon ve Kuplaj Reaksiyonu ile Saf Form ve Farmasötik formülasyonlarda Mesalazin'in spektrofotometrik tayini

An accurate, simple, and precise spectrofluorimetric method is presented for the determination of Mesalazine (MZN) based on Diazotization in acidic medium through reaction it with Sodium nitrite (NaNO2) to result diazonium salt that Coupling Reaction in medium base with 2,7-Dihydroxynaphthalene (DHNP) to formed azo dye. The intensity of the dye was measured at 501 nm after optimization of the experimental parameters. Beer’s law was applied to the proposed method and it was valid within a concentration range of 0.25–10 μg/mL and the linear regression was R2 = 0.9974. The limit of quantitation was 0.0241 μg/mL, and the molar absorptivity coefficient 1.85x104 L.mol−1.cm−1. Sandal's sensitivity was 0.0082 μg.cm-2 There is no interference from excipients found in the tablet. The data were statistically compared with British Pharmacopoeia methods asstandard reference using Student's t-and F-test.