{"title":"嗜热纤维素真菌β-葡萄糖苷酶的定位与释放","authors":"Joel Solomon Gaikwad , Ramesh Maheshwari","doi":"10.1016/S0147-5975(06)80003-4","DOIUrl":null,"url":null,"abstract":"<div><p>In a medium containing cellulose as the carbon source, the rapid growth of <em>Sporotrichum thermophile</em>, the secretion of cellulases and the utilization of cellulose were well-correlated events. The production of β-glucosidase in culture medium lagged behind cellulases, coinciding with the time of extensive autolysis of mycelia. By contrast, neither apparent autolysis nor secretion of β-glucosidase occurred when <em>S. thermophile</em> was grown in medium containing cellobiose; the enzyme activity remained associated with mycelia. The release of β-glucosidase in cellulose-grown cultures was correlated with the activity of the lytic enzyme in the cell wall. Immunocytochemical localization and biochemical characterization showed that a β-glucosidase released in the cellulose medium was the same as that which remained associated with mycelia grown on cellobiose. The results indicated that the release of β-glucosidase in the cellulose culture is incidental to the activity of the lytic enzymes which are strongly induced by cellulose. The observations minimize a functional role of the culture fluid P-glucosidase in cellulolysis by the fungus. Rather, the available information suggests that the cellulases and β-glucosidases associated with the hyphal cell wall may play a role in cellulolysis by the fungus.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"18 4","pages":"Pages 300-310"},"PeriodicalIF":0.0000,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0147-5975(06)80003-4","citationCount":"17","resultStr":"{\"title\":\"Localization and release of β-glucosidase in the thermophilic and cellulolytic fungus, Sporotrichum thermophile\",\"authors\":\"Joel Solomon Gaikwad , Ramesh Maheshwari\",\"doi\":\"10.1016/S0147-5975(06)80003-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In a medium containing cellulose as the carbon source, the rapid growth of <em>Sporotrichum thermophile</em>, the secretion of cellulases and the utilization of cellulose were well-correlated events. The production of β-glucosidase in culture medium lagged behind cellulases, coinciding with the time of extensive autolysis of mycelia. By contrast, neither apparent autolysis nor secretion of β-glucosidase occurred when <em>S. thermophile</em> was grown in medium containing cellobiose; the enzyme activity remained associated with mycelia. The release of β-glucosidase in cellulose-grown cultures was correlated with the activity of the lytic enzyme in the cell wall. Immunocytochemical localization and biochemical characterization showed that a β-glucosidase released in the cellulose medium was the same as that which remained associated with mycelia grown on cellobiose. The results indicated that the release of β-glucosidase in the cellulose culture is incidental to the activity of the lytic enzymes which are strongly induced by cellulose. The observations minimize a functional role of the culture fluid P-glucosidase in cellulolysis by the fungus. Rather, the available information suggests that the cellulases and β-glucosidases associated with the hyphal cell wall may play a role in cellulolysis by the fungus.</p></div>\",\"PeriodicalId\":12110,\"journal\":{\"name\":\"Experimental Mycology\",\"volume\":\"18 4\",\"pages\":\"Pages 300-310\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0147-5975(06)80003-4\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147597506800034\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597506800034","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Localization and release of β-glucosidase in the thermophilic and cellulolytic fungus, Sporotrichum thermophile
In a medium containing cellulose as the carbon source, the rapid growth of Sporotrichum thermophile, the secretion of cellulases and the utilization of cellulose were well-correlated events. The production of β-glucosidase in culture medium lagged behind cellulases, coinciding with the time of extensive autolysis of mycelia. By contrast, neither apparent autolysis nor secretion of β-glucosidase occurred when S. thermophile was grown in medium containing cellobiose; the enzyme activity remained associated with mycelia. The release of β-glucosidase in cellulose-grown cultures was correlated with the activity of the lytic enzyme in the cell wall. Immunocytochemical localization and biochemical characterization showed that a β-glucosidase released in the cellulose medium was the same as that which remained associated with mycelia grown on cellobiose. The results indicated that the release of β-glucosidase in the cellulose culture is incidental to the activity of the lytic enzymes which are strongly induced by cellulose. The observations minimize a functional role of the culture fluid P-glucosidase in cellulolysis by the fungus. Rather, the available information suggests that the cellulases and β-glucosidases associated with the hyphal cell wall may play a role in cellulolysis by the fungus.