南极细菌中的不饱和脂肪酸

L. Garba, W. Latip, M. Ali, Siti Nurbaya Oslan, R. Rahman
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引用次数: 7

摘要

不饱和脂肪酸(UFAs)在维持膜脂结构完整性的流动性中起着至关重要的作用,而且具有重要的营养和药用价值。鱼油和植物油是人体正常生理活动所必需的所谓必需脂肪酸的主要来源。然而,对这些脂肪酸日益增长的需求需要更可靠、更灵活和更便宜的替代来源。南极细菌可以产生大量的不饱和脂肪酸,这可以通过简单的生物技术工具来增强。为了实现这一目标,研究人员在低温下培养了5株分离的南极细菌,并使用气相色谱-质谱法(GCMS)分析了细胞脂肪酸。结果显示,几乎所有的细菌都含有大量的不饱和脂肪酸。其中以3B节杆菌最多(47.24%),其次为A8假单胞菌(45.09%)、A3假单胞菌(33.17%)和PB节杆菌(31.92%)。结果表明,该细菌可通过代谢工程技术提高必需脂肪酸的产量。
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Unsaturated fatty acids in antarctic bacteria
Unsaturated Fatty Acids (UFAs) play a vital role in membrane lipids fluidity which maintains its structural integrity and also, are nutritionally and pharmaceutically important. Fish and vegetable oils are the major sources of the so-called essential fatty acids required for normal physiological activities of the body. However, the increasing demands for these fatty acids require more reliable, flexible and cheaper alternative sources. Antarctic bacteria can produce large amounts of unsaturated fatty acids which can be enhanced using simple biotechnological tools. To achieve this, five isolates of Antarctic bacteria were grown at low temperature and analysed for cellular fatty acids using Gas Chromatography Mass Spectrometry (GCMS). The results obtained revealed high amount of unsaturated fatty acids in virtually all the bacteria. Highest amount was recorded in Arthrobacter sp., 3B (47.24%) followed by Pseudomonas sp., A8 (45.09%), Pseudomonas sp., A3 (33.17%) and Arthrobacter sp., PB (31.92%). The results obtained suggest that the bacteria could be used to enhance essential fatty acids production through metabolic engineering for industrial applications.
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