玛咖超临界提取物中的抗衰老活性物质 Anti-Aging Substances from Maca Supercritical Extract

本研究采用酵母突变株K6001作为生物活性筛选系统，对韩国圃美多公司多个产品进行筛选，发现玛咖超临界提取粉末具有较好的抗衰老活性。因此对该原料中的抗衰老活性物质进行了研究并从中分离得到了两个活性化合物(1, 2)。这两个化合物都能显著延长酵母突变株的复制性寿命。通过波谱分析以及文献数据比对，推测活性化合物1的结构为n-benzyl-(9Z)-octadecenamide(1)，确定活性化合物2的结构为n-benzylmyristamide(2)。为了进一步确认化合物1的结构，合成了n-benzyl-(9Z)-octadecena- mide(1)，并且测定了其生物活性，结果表明合成得到的n-benzyl-(9Z)-octadecenamide的波谱数据及生物活性与天然来源的化合物1相同。 Extracts of several products of Pulmuone were screened for their anti-aging activity using the K6001 yeast strain bioassay system. Maca supercritical extract significantly exhibited anti-aging activity and two active compounds were isolated from the extract. The structures were elucidated by the spectroscopic data and comparison with those reported. One structure was proposed to be n-benzyl-(9Z)-octadecenamide (1), and another was identified as n-benzylmyristamide (2). Both compounds significantly extended the replicative lifespan of K6001 yeast strain. To confirm the structure of compound 1, n-benzyl-(9Z)-octadecenamide (1) was synthesized and biological activity was evaluated. The synthesized n-benzyl-(9Z)-octadecenamide (1) is identical to natural compound 1 from spectroscopic data and biological activity.