Growth and Development of Shoot on Lime (Citrus hystrix)

Citrus hystrixis a famous horticutural crops product today. Fresh fruits of Citrus hystrix from Indonesia are mainly exported to European countries by PT Nusantara Segar Global under the trademark of Java Fresh. Futhermore, the seedlings of Citrus hystrix were also used to be a rootstock for RGL citrus due to its vigorous root growth, adataptive to marginal land, and resistance to dry season. The objective of research was to determine the effects of difference kind and concentration of cytokinin to induce shoot of Citrus hystrix. The research was conducted from January to April 2019 at the Laboratory of Biotechnology and Tissue Culture, Faculty of Agriculture, Bengkulu University. The research was designed to be Completely Randomized Block Design (CRBD). First factor was kind of cytokinin (BAP and Kinetin). Second factor was the concentration of cytokinins (0 ppm, 1 ppm, 2 ppm, 3 ppm, 4 ppm, and 5 ppm). Therefore, there were 12 combinations with 3 replications. The results show, the number of shoot leaf of Citrus hystrix shows a different respon to the type of cytokinin. The best type of cytokinin in the shoot of Citrus hystrix was BAP, that produced the highest number of leaves, the highest number of shoots and the heaviest wet weight of shoots, namely 10.42 leaves/bottle, 3.2 shoots and 0.46 gr. The optimum concentration of BAP was 2.58 ppm.