集成微流体的芯片上电化学免疫传感器阵列:开发和表征

Shifa Felemban, P. Vazquez, T. Balbaied, E. Moore
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引用次数: 1

摘要

芯片实验室最近因其便携性和与电化学免疫分析的简单集成而成为原位监测的替代方案。在这里,我们提出了一个电化学电池芯片配置在一个三电极系统,以检测水中苯并(a)芘(BaP)。11-巯基十四酸(MUA)是一种自组装单层(SAM),用于修饰金芯片表面,以降低抗体结合的随机性。在抗体固定前,用-乙基-3-(3-二甲氨基丙基)(EDC)与n -氢琥珀酰亚胺(NHS)联合激活一个羧酸基团。采用循环伏安法和接触角测量法研究了金表面电化学反应和SAM形成的机理。数据表明,改进后的芯片具有较低的接触角和50 mV/s的扫描速率。通过在SAM中添加修饰层和硫醇端基,我们的设计使芯片表面变得更加绝缘。采用开发的q感系统进行安培检测。该新技术检测了多个样品,并相当快地完成了分析。虽然综合系统在实验室环境中证明是成功的,但研究的目的是将该系统用于现场分析,可以将其带入水环境中,以现有工艺进行测试。通过这种方式,可以有效地纠正环境设置可能产生的任何问题。
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Lab-on-a-Chip Electrochemical Immunosensor Array Integrated with Microfluidics: Development and Characterisation
Lab-on-a-chip has recently become an alternative for in situ monitoring for its portability and simple integration with an electrochemical immunoassay. Here, we present an electrochemical cell-on-a-chip configured in a three-electrode system to detect benzo(a)pyrene (BaP) in water. 11-Mercaptoundecanoic acid (MUA), a self-assembled monolayer (SAM), was used to modify a gold chip surface to reduce the randomness of antibody binding. A carboxylic acid group was activated with -ethyl-3-(3-dimethylaminopropyl) (EDC) in combination with N-hyrodsuccinimide (NHS) before antibody immobilisation. The mechanism of the electrochemical reactions on a gold surface and SAM formation were investigated by cyclic voltammetry and contact angle measurements. The data revealed a lower contact angle in the modified chip and a scan rate of 50 mV/s. Through the addition of modification layers and thiol end groups to the SAM, our design allowed the chip surface to became more insulated. All were tested by amperometric detection using the developed Q-sense system. This novel technique detected multiple samples, and completed the analysis reasonably quickly. While the integrated system proved successful in a lab setting, the aim of the research is to use this system for in situ analysis, which can be brought into a water environment to carry out tests with existing processes. In this way, any issues that may arise from an environmental setting can be rectified in an efficient manner.
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