编码血清诱导激酶的早期生长反应基因Snk的人类同源物的鉴定

K. Liby, Huiyun Wu, B. Ouyang, Shecao Wu, Jie Chen, W. Dai
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引用次数: 29

摘要

小鼠血清诱导激酶(mSnk)是最近克隆的一种参与细胞增殖的早期生长反应基因。在这里,我们报道了其人类同源物hSnk的分离和鉴定。序列比较表明,hSnk具有高度保守性,推导出的hSnk蛋白序列与mSnk、rSnk蛋白以及其他polo家族激酶基因产物具有显著的氨基酸同源性。一项对hSnk表达的调查显示,尽管多种人体组织表达低至中等水平的hSnk转录本,但胎儿组织、睾丸和脾脏表达最丰富的hSnk转录本。此外,血清刺激可迅速诱导成纤维细胞中hSnk的表达,并在处理后1小时内达到诱导峰值。考虑到Plk和Prk,另外两个已知的人类polo家族激酶,控制细胞周期检查点和细胞周期进程,我们目前的观察表明,hSnk也可能在细胞快速分裂或具有高有丝分裂指数的细胞中发挥重要作用。
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Identification of the Human Homologue of the Early-Growth Response Gene Snk, Encoding a Serum-Inducible Kinase
Murine serum inducible kinase (mSnk) was recently cloned and characterized as an early-growth response gene involved in cell proliferation. Here we report the isolation and characterization of its human homologue, named hSnk. Sequence comparison shows that hSnk is highly conserved and its deduced protein sequence shares a significant amino acid identity with mSnk and rSnk proteins, as well as with other polo family kinase gene products. A survey ofhSnk expression reveals that while a wide variety of human tissues express a low to moderate level of hSnk transcripts, fetal tissues, testis, and spleen express the most abundant hSnk transcripts. In addition, serum stimulation rapidly induces hSnk expression in fibroblast cells, reaching the peak level of induction within one hour post treatment. Considering that Plk and Prk, two other known human polo-family kinases, control cell cycle checkpoint and cell cycle progression, our current observations suggest that hSnk may also play an important role in cells undergoing rapid cell division or having a high mitotic index.
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